Antifungal Peptides from a Burkholderia Strain Suppress Basal Stem Rot Disease of Oil Palm

Author:

Prihatna Cahya1ORCID,Pramudito Theodorus Eko12,Arifin Arild Ranlym1ORCID,Nguyen Thi Kim Ngan3,Purnamasari Maria Indah1,Suwanto Antonius4ORCID

Affiliation:

1. Research and Development for Biotechnology, PT Wilmar Benih Indonesia, Bekasi, Jawa Barat, Indonesia 17530

2. Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, Tangerang, Indonesia 15345

3. WIL@NUS Corporate Lab, Singapore 117599

4. Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University. Bogor, Jawa Barat, Indonesia 16680

Abstract

Basal stem rot (BSR) is the most common disease of oil palm (Elaeis guineensis) in Southeast Asia. BSR is caused by a white-rot fungus Ganoderma boninense. The disease is difficult to manage. Therefore, development of novel and environmentally safe approaches to control the disease is important. Species of Burkholderia are known to have diverse lifestyles, some of which can benefit plants by suppressing diseases or increasing plant growth. In the present study, antifungal peptides produced by a bacterial strain isolated from the rhizosphere of an oil palm tree, Burkholderia sp. strain CP01, exhibited strong growth inhibition on G. boninense. A loss-of-function mutant of CP01 was generated, and it has enabled the identification of a 1.2-kDa peptide and its variants as the active antifungal compounds. High-resolution mass spectrometry revealed six analogous compounds with monoisotopic masses similar to the previously reported cyclic lipopeptides occidiofungin and burkholdine. The antifungal compounds of CP01 were secreted into media, and we sought to use CP01 culture extract without living cells to control BSR disease. Glasshouse experiments showed that CP01 culture extract suppressed BSR disease in oil palm seedlings. The ability of CP01 to produce an antifungal substance and suppress plant disease suggests its potential applications as a biofungicide in agriculture.

Publisher

Scientific Societies

Subject

Plant Science,Agronomy and Crop Science

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