Identifying Characteristics of Verticillium Wilt Suppressiveness in Olive Mill Composts

Abstract

The aims of this study were to assess the potential suppressive effects of different olive mill composts on Verticillium wilt and to elucidate the suppressive mechanisms. To this end, four olive mill composts from different crop areas with two maturation levels were selected. After conducting the Verticillium wilt bioassays in cotton, the suppressive effect was observed in only one compost. Compost maturation level did not affect disease development. The standardized area under the disease progress curve and microsclerotia concentration were associated with low API-ZYM enzymatic diversity, β-glucosidase activity, pH, and high electrical conductivity (EC). To assess the nature of suppressiveness in the suppressive compost, additional bioassays were performed with three treated compost-amended growing media (N-supplemented, autoclaved, and heat treated at 60°C for 6 days). Suppressiveness was partially reduced with heat treatments, where N-acetyl-β-glucosaminidase activity disappeared. In this compost, high oligotrophic actinomycete populations were associated with disease reduction. Therefore, plant growth media amended with different olive mill composts do not always show suppressiveness against Verticillium wilt. Enzymatic diversity, β-glucosidase activity, pH, and EC may be sufficient to predict where olive mill compost plant growth media will be effective in reducing Verticillium wilt and microsclerotia concentration. General and specific suppressiveness are involved in the mechanism of compost suppression.