Antagonistic Activity of Bacillus velezensis SDTB038 against Phytophthora infestans in Potato

Abstract

Potato late blight is a severe and highly epidemic disease caused by Phytophthora infestans that can affect all parts of the plant. This study mainly screened antagonistic strains for good control of potato late blight and identified strain SDTB038 as Bacillus velezensis according to its morphological and chemical properties and the 16S rRNA, gyrA, and gyrB gene sequences. This antagonistic strain achieved good control of potato late blight in greenhouses and fields and promoted potato plant growth. Two-year field trials (2018 and 2019) showed that B. velezensis SDTB038 can be used to reduce food losses caused by late blight, achieving late blight reductions of 40.79% (2018) and 37.67% (2019). In two-year field trials, the control effects of the highest concentrations of fluopimomide and B. velezensis SDTB038 were better than those of the other treatments. The control effect of 85 g ha−1 fluopimomide and B. velezensis SDTB038 and that of 170 g ha−1 fluopimomide alone showed no significant differences. These field results indicate that a low concentration of fungicide and a high concentration of SDTB038 can be effective in controlling potato late blight. Foliar detection showed that lipopeptides have an inhibitory effect on P. infestans. The amplification of lipopeptide genes revealed surfactin (srfAB and srfAC) and fengycin (fenB) genes in SDTB038, but only surfactin production by B. velezensis SDTB038 was observed by ultra-high-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry analysis. Therefore, the strain B. velezensis SDTB038 can produce secondary metabolites that help potato plants resist late blight development, can effectively inhibit the infection of potato leaves by P. infestans, and has potential value for development as a biological pesticide against potato late blight.