Direct Evidence Demonstrates that Puccinia striiformis f. sp. tritici Infects Susceptible Barberry to Complete Sexual Cycle in Autumn

Author:

Du Zhimin1,Peng Yuelin2,Zhang Gensheng1ORCID,Chen Li3,Jiang Shuchang1,Kang Zhensheng1ORCID,Zhao Jie1ORCID

Affiliation:

1. State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China

2. Department of Plant Sciences, Agricultural and Animal Husbandry College of Tibet University, Linzhi, Tibet 86000, China

3. Extension Center for Agricultural Technology, Agriculture Department of Tibetan Autonomous Region, Tibet, China

Abstract

Wheat stripe rust is an airborne and destructive disease caused by a heteroecious rust fungus Puccinia striiformis f. sp. tritici (Pst). Studies have demonstrated that the rust pathogen accomplishes sexual reproduction on susceptible barberry under natural conditions in spring, whereas Pst infection on barberry is still in blank in other seasons. In late October 2016, aecial production on barberry shrubs were observed in Linzhi, Tibet, China. Therefore, experimental tests were conducted to verify the existence of sexual cycles of Pst in this season. By inoculating 52 aecial clusters from 30 rusted barberry leaves, four Pst samples, T1 to T4, were successfully recovered from the rusted barberry shrubs. Sixty-five single uredinium (SU) isolates were derived from the four Pst samples. Based on virulence tests on the Chinese differential hosts, T1 to T4 samples were unknown races and showed mixed reactions on some differentials. Twenty-one known races and 44 unknown races belonging to five race groups were identified among the 65 SU isolates. Meanwhile, the 65 SU isolates produced 26 various virulence patterns (VPs; called VP1-VP26) on 25 single Yr gene lines and 15 multilocus genotypes (MLGs) at nine simple sequence repeat marker loci. Clustering analysis showed similar lineage among subpopulations and different lineage between subpopulations. Linkage disequilibrium analysis indicated that the SU population was produced sexually. This study first reported that Pst infects susceptible barberry to complete sexual reproduction in autumn. The results update the knowledge of disease cycle and management of wheat stripe rust and contribute to the understanding of rust genetic diversity in Tibet.

Funder

National Key R&D Program of China

National Natural Science Foundation of China

Natural Science Basic Research Plan in Shaanxi Province of China

Key Project of Science and Technology of Tibetan Autonomous Region, China

National “111 Plan”

Publisher

Scientific Societies

Subject

Plant Science,Agronomy and Crop Science

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