Selection of Differential Isolates of Magnaporthe oryzae for Postulation of Blast Resistance Genes

Author:

Fang W. W.1,Liu C. C.1,Zhang H. W.1,Xu H.1,Zhou S.1,Fang K. X.1,Peng Y. L.1,Zhao W. S.1

Affiliation:

1. First, second, third, fifth, sixth, seventh, and eighth authors: State Key Laboratory of Agrobiotechnology and MOA Key Lab of Pest Monitoring and Green Management, China Agricultural University, Beijing 100193, China; and fourth author: Institute of Plant Protection, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China.

Abstract

A set of differential isolates of Magnaporthe oryzae is needed for the postulation of blast resistance genes in numerous rice varieties and breeding materials. In this study, the pathotypes of 1,377 M. oryzae isolates from different regions of China were determined by inoculating detached rice leaves of 24 monogenic lines. Among them, 25 isolates were selected as differential isolates based on the following characteristics: they had distinct responses on the monogenic lines, contained the minimum number of avirulence genes, were stable in pathogenicity and conidiation during consecutive culture, were consistent colony growth rate, and, together, could differentiate combinations of the 24 major blast resistance genes. Seedlings of rice cultivars were inoculated with this differential set of isolates to postulate whether they contain 1 or more than 1 of the 24 blast resistance genes. The results were consistent with those from polymerase chain reaction analysis of target resistance genes. Establishment of a standard set of differential isolates will facilitate breeding for blast resistance and improved management of rice blast disease.

Funder

Ministry of Agriculture of the People's Republic of China

National Rice Industry System of China

Publisher

Scientific Societies

Subject

Plant Science,Agronomy and Crop Science

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