First Expansion of the Public Tomato Brown Rugose Fruit Virus (ToBRFV) Nextstrain Build; Inclusion of New Genomic and Epidemiological Data

Abstract

Tomato brown rugose fruit virus (ToBRFV) is a tobamovirus that was first detected in Israel and Jordan following an outbreak of a new disease infecting tomato in 2014. Since then, the virus has been reported from all continents except Oceania and Antarctica. In response to the first finding of the virus in The Netherlands, the Dutch National Plant Protection Organization created a ToBRFV Nextstrain build (v1). In this report, we announce 47 new (near) complete ToBRFV genomes and the generation of the new ToBRFV Nextstrain (v2) build containing 118 ToBRFV genomes with associated geographic and epidemiological data. Examples of utilization of the genomic sequences are presented, and we report the first sequence from South America and present a novel hypothesis on the possible ToBRFV center of origin. [Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .