Characteristics of Xanthomonas cucurbitae Isolates from Pumpkins and Survival of the Bacterium in Pumpkin Seeds

Abstract

This study was conducted to determine characteristics of Xanthomonas cucurbitae, the causal agent of bacterial spot of pumpkin, and survival of the bacterium in pumpkin seeds. Fourteen X. cucurbitae isolates from the north central region of the United States, along with the X. cucurbitae strain 23378 from the American Type Culture Collection, were included in this study. The range of minimum, optimum, and maximum temperatures for colony development of X. cucurbitae were 4 to 6°C, 24 to 30°C, and 34 to 36°C, respectively. Optimum pH for colony development ranged from 6.5 to 8.0. Leaves of 3-week-old pumpkins ‘Howden’ and ‘Dickinson’ were inoculated with X. cucurbitae isolates (108 CFU/ml). There was a significant difference in the postinoculation periods for appearance of bacterial lesions on the leaves among the isolates; however, there was no significant difference in diameters of the lesions on each of the pumpkin cultivar 7 days after inoculation. Four of the isolates caused significantly larger lesions on ‘Dickinson’ leaves than ‘Howden’ leaves. Naturally infected ‘Howden’ pumpkin and inoculated ‘Dickinson’ pumpkin seeds with X. cucurbitae were stored at 4 and 22°C. X. cucurbitae was isolated from both naturally infected and inoculated seeds 24 months after storage at both 4 and 22°C, and the isolated bacteria were pathogenic.