Combined use of phenotype-based and genome-informed approaches identified a unique Fusarium oxysporum f. sp. cubense isolate in Hawaii

Abstract

Fusarium wilt of banana, caused by Fusarium oxysporum f. sp. cubense (Foc), is a serious disease that threatens banana production worldwide. It is a long-standing problem in Hawaii, but there was little knowledge of the causal pathogen. We isolated a strain of Foc, named Foc-UH, from a field experiencing the disease epidemic in Hawaii. Infection assays of a diverse panel of 26 banana clones, including varieties used for differentiating pathogen races and fruit production, revealed Foc-UH has a race 1 pathogenic phenotype with an intermediate race 2 virulence, and revealed the differential resistance of varieties to infection. Separate phylogenetic analyses using the barcoding regions of three nuclear genes, seven complete nuclear genes, and single nucleotide polymorphisms within conserved whole genome protein coding sequences, placed Foc-UH into recently proposed taxonomic frameworks relevant to Foc and the Fusarium oxysporum species complex. Screening of the 99.7% complete draft genome identified five secreted in xylem (SIX) gene homologs, including SIX1d, SIX1f, SIX9a, SIX9b, and SIX13a. This profile is similar to that of several race 1 isolates except the absence of SIX4 and SIX6. Foc-UH was morphologically dissimilar to the nearest related isolates. Altogether, this study identified a unique isolate that causes banana Fusarium wilt, which represents the first characterization of the causal pathogen in Hawaii. The findings and the genomic resources generated in this study are expected to guide banana breeding and cultivar deployment in Hawaii and beyond, and contribute to further understanding of the pathogenicity and evolutionary systematics of Foc.