Grassy Weeds and Corn as Potential Sources of Barley yellow dwarf virus Spread Into Winter Wheat

Abstract

Barley yellow dwarf virus (BYDV) is an important vector-borne pathogen of cereals. Although many species of grasses are known to host BYDV, knowledge of their role in virus spread in regional agroecosystems remains limited. Between 2012 and 2016, Idaho winter wheat production was affected by BYDV. BYDV-PAV and the bird cherry-oat aphid (BCOA) (Rhopalosiphum padi L.) vector were commonly present in the affected areas. A series of greenhouse bioassays were performed to examine whether two types of corn (Zea mays L.), dent and sweet, and three commonly found grassy weeds, downy brome (Bromus tectorum L.), green foxtail (Setaria viridis L.), and foxtail barley (Hordeum jubatum L.), can be inoculated with BYDV (species BYDV-PAV) by BCOA and also act as sources of the virus in winter wheat. BCOA successfully transmitted BYDV-PAV to both corn types and all weed species. Virus titers differed between the weed species (P = 0.032) and between corn types (P = 0.001). In transmission bioassays, aphids were able to survive on these host plants during the 5-day acquisition access period and later successfully transmitted BYDV-PAV to winter wheat (var. SY Ovation). Transmission success was positively correlated with the virus titer of the source plant (P < 0.001) and influenced by weed species (P = 0.028) but not corn type. Overall, the results of our inoculation and transmission assays showed that the examined weed species and corn types can be inoculated with BYDV-PAV by BCOA and subsequently act as sources of infections in winter wheat.