Ethyl Acetate Extract of Radix Cynanchi Auriculati Exerts Antioxidant Effects on LPS-induced RAW264.7 Cells by Regulating MAPKs/Nrf2/HO-1 Pathways-Reference-Cited by-同舟云学术

Ethyl Acetate Extract of Radix Cynanchi Auriculati Exerts Antioxidant Effects on LPS-induced RAW264.7 Cells by Regulating MAPKs/Nrf2/HO-1 Pathways

Published:2023-03-27 Issue:2 Volume:5 Page:144-148
ISSN:2771-3032
Container-title:Academic Journal of Science and Technology
language:
Short-container-title:AJST

Author:

Ren Jie,Su Ya,Zeng Lili,Zhou Jichao,Yi Siqi,Xing Yi,Hu Kun

Abstract

Extracts of Radix Cynanchi Auriculati (RCA) have multiple effects. However, it remains unclear whether RCA extracts can exhibit antioxidant activity by regulating MAPKs/Nrf2/HO-1 pathways. In this study, we evaluated the antioxidant activity of ethyl acetate extract of RCA (RCAEA) via LPS-induced RAW264.7 cells, and investigated the potential mechanism of its protective effect against oxidative damage on macrophages. The results showed that RCAEA activating MAPKs/Nrf2/HO-1 pathways. In conclusion, the aim of this study was to describe the molecular mechanisms responsible for the RCAEA mediating the inhibitory effects of oxidative stress in RAW264.7 cells.

Publisher

Darcy & Roy Press Co. Ltd.

Reference17 articles.

1. Bellezza, I., Giambanco I., Minelli A. and Donato R. Nrf2-Keap1 signaling in oxidative and reductive stress. Biochim Biophys Acta Mol Cell Res 1865: 721-733, 2018.

2. Bryan, H.K., Olayanju A., Goldring C.E. and Park B.K. The Nrf2 cell defence pathway: Keap1-dependent and -independent mechanisms of regulation. Biochem Pharmacol 85: 705-717, 2013.

3. Cargnello, M. and Roux P.P. Activation and function of the MAPKs and their substrates, the MAPK-activated protein kinases. Microbiol Mol Biol Rev 75: 50-83, 2011.

4. Chen, H.L., Jia W.J., Li H.E., Han H., Li F., Zhang X.L., Li J.J., Yuan Y. and Wu C.Y. Scutellarin Exerts Anti-Inflammatory Effects in Activated Microglia/Brain Macrophage in Cerebral Ischemia and in Activated BV-2 Microglia Through Regulation of MAPKs Signaling Pathway. Neuromolecular Med 22: 264-277, 2020.

5. Johnson, G.L. and Lapadat R. Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases. Science 298: 1911-1912, 2002.