Application Of CRISPR-Cas in Pathogen Detection

Abstract

Infectious diseases have had a significant influence on the market economy and human civilization in recent years. Traditional nucleic acid tests typically include a lot of procedures, are complicated, take a long time to complete, and have significant space, staff, and equipment needs. More and more study is being done in this area as a result of the identification of clustered regularly interspaced short palindromic repeats and related(CRISPR-Cas) proteins in prokaryotes. The CRISPR-Cas system has been extensively used to detect viruses that cause infectious diseases due of its high specificity and sensibility. The history of the CRISPR-Cas system, including its revised categorization, is discussed in this paper. Besides, a summary of the use of various CRISPR-Cas system types in pathogen detection is also provided in the paper, along with an introduction to the CRISPR-Cas system’s basic principles employing CRISPR-Cas9 as an example. As a biosciences frontier hot spot, research on CRISPR-Cas systems is developing quickly and technology based on the CRISPR-Cas system for detecting pathogens is extremely useful in clinical diagnosis and biological research.