The SCFAs Production of Syntrophic Culture of L. johnsonii SZ-YL and A. Muciniphila in Different Macrobutrients

Abstract

The gut microbiota is a complex ecological system that influences numerous host physiology, such as obesity, appetite, immunity and drug metabolism. As a crucial component in intestine, short chain fatty acids (SCFAs) can modulate host metabolism and disease physiology via receptor, such as GPCRs. The system of knowledge of the host gut microbiota has been greatly expanded. Diets alterations can rapidly modify gut microbial composition and affect SCFAs production. Current knowledge of SCFAs production is mainly based on isolates or intestinal environment, but these may not reflect the effects of bacterial interaction on SCFAs generation. In these studies, the potential effects and SCFAs production of in a syntrophic coculture of L. johnsonii and A. muciniphila was investigated in different medium. Cocultures produced higher acetate and propionate in glucose and inulin medium. In oleic acid addition medium, the growth of L. johnsonii were promoted and the acetate production were promoted. These results indicate that appropriate diet proportion is necessary for beneficial bacteria growth to affect host health. Moreover, unsaturated fatty acid promotes the growth of Lactobacillus instead of A. muciniphila. Our results indicated that the changes of diet proportion might be potential method for beneficial bacteria growth.