Author:
Ye Ruixi,Chen Xi,Li Yutong
Abstract
CRISPR/Cas systems were novel multifunctional genome editing techniques, and the modification of Cas proteins endowed them with more efficient and precise properties. Herein, we systematically outlined the principles and applications of CRISPR/Cas systems in disease prevention, mechanism research, and gene treatment of diseases. Specifically, we detailed the systems widely utilized, containing the Cas9, Cas12a, and Cas13a systems, and summarized the current status of the medical applications of these systems in terms of vaccine design, trait improvement, animal models of human diseases, organ transplantation, gene therapy, and gene detection. We concluded that CRISPR/Cas systems had great potential to advance medical research and practice but also should be carefully regulated and supervised to ensure their safety and efficacy.
Publisher
Darcy & Roy Press Co. Ltd.
Reference25 articles.
1. Gostimskaya I. CRISPR–Cas9: A History of Its Discovery and Ethical Considerations of Its Use in Genome Editing [J]. Biochemistry (Moscow), 2022, 87 (8): 777 - 788.
2. KKoonin E V, Makarova K S. Origins and evolution of CRISPR-Cas systems [J]. Philosophical Transactions of the Royal Society B, 2019, 374 (1772): 20180087.
3. Sergey Shmakov, Omar O Abudayyeh, Kira S Makarova, et al. Discovery and functional characterization of diverse Class 2 CRISPR-Cas systems [J]. Mol Cell, 2015, 60 (3): 385 - 397.
4. Zhao Kexin, Liu Yingying, Ma Xuezheng, et al. Study of rapid and visual detection method for SARS-CoV-2based on CRISPR-Cas13a system [J]. Chinese Journal of Frontier Health and Quarantine, 2022, 45 (04): 257 - 259.
5. Su Xuan, Ge Yiyue, Zhang Qian,et al.Rapid detection of Staphylococcus aureus by CRISPR-Cas13a combined with recombinase aided amplification (RAA) [J]. Journal of Pathogen Biology, 2020, 15 (03): 253 - 258.