Author:
Cai Jingli,Olson Judith M,Rao Mahendra S,Stanley Marisa,Taylor Eva,Ni Hsiao-Tzu
Abstract
Abstract
Background
Using antibodies to specific protein antigens is the method of choice to assign and identify cell lineage through simultaneous analysis of surface molecules and intracellular markers. Embryonic stem cell research can be benefited from using antibodies specific to transcriptional factors/markers that contribute to the "stemness" phenotype or critical for cell lineage.
Results
In this report, we have developed and validated antibodies (either monoclonal or polyclonal) specific to human embryonic stem cell antigens and early differentiation transcriptional factors/markers that are critical for cell differentiation into definite lineage.
Conclusion
These antibodies enable stem cell biologists to conveniently identify stem cell characteristics and to quantitatively assess differentiation.
Publisher
Springer Science and Business Media LLC
Cited by
15 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献