Author:
Link Vinzenz,Shevchenko Andrej,Heisenberg Carl-Philipp
Abstract
Abstract
Background
Zebrafish (D. rerio) has become a powerful and widely used model system for the analysis of vertebrate embryogenesis and organ development. While genetic methods are readily available in zebrafish, protocols for two dimensional (2D) gel electrophoresis and proteomics have yet to be developed.
Results
As a prerequisite to carry out proteomic experiments with early zebrafish embryos, we developed a method to efficiently remove the yolk from large batches of embryos. This method enabled high resolution 2D gel electrophoresis and improved Western blotting considerably. Here, we provide detailed protocols for proteomics in zebrafish from sample preparation to mass spectrometry (MS), including a comparison of databases for MS identification of zebrafish proteins.
Conclusion
The provided protocols for proteomic analysis of early embryos enable research to be taken in novel directions in embryogenesis.
Publisher
Springer Science and Business Media LLC
Reference22 articles.
1. Westerfield M: The zebrafish book. A guide for the laboratory use of zebrafish (Danio rerio). 2000, Eugene, Univ. of Oregon Press, 4
2. Nüsslein-Volhard C, Dahm R: Zebrafish : a practical approach. 2002, Oxford ; New York, Oxford University Press, xviii, 303 , [8] of plates-1st
3. Love DR, Pichler FB, Dodd A, Copp BR, Greenwood DR: Technology for high-throughput screens: the present and future using zebrafish. Curr Opin Biotechnol. 2004, 15: 564-571. 10.1016/j.copbio.2004.09.004.
4. Bosworth CA, Chou CW, Cole RB, Rees BB: Protein expression patterns in zebrafish skeletal muscle: initial characterization and the effects of hypoxic exposure. Proteomics. 2005, 5: 1362-1371. 10.1002/pmic.200401002.
5. Shrader EA, Henry TR, Greeley MSJ, Bradley BP: Proteomics in zebrafish exposed to endocrine disrupting chemicals. Ecotoxicology. 2003, 12: 485-488. 10.1023/B:ECTX.0000003034.69538.eb.
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