Author:
Ivanova Anna,Wuerfel Jens,Zhang Juan,Hoffmann Olaf,Ballmaier Matthias,Dame Christof
Abstract
Abstract
Background
Thrombopoietin (Thpo) and its receptor (Mpl), which regulate megakaryopoiesis, are expressed in the central nervous system (CNS), where Thpo is thought to exert pro-apoptotic effects on newly generated neurons. Mpl expression has been analysed in brain tissue on transcript level and in cultured primary rat neurons and astrocytes on protein level. Herein, we analysed Mpl expression in the developing and adult murine CNS by immunohistochemistry and investigated the brain of mice with homozygous Mpl deficiency (Mpl
-/-) by MRI.
Results
Mpl was not detectable at developmental stages E12 to E15 in any resident cells of the CNS. From E18 onwards, robust Mpl expression was found in various brain areas, including cerebral cortex, olfactory bulb, thalamus, hypothalamus, medulla, pons, and the grey matter of spinal cord. However, major developmental changes became obvious: In the subventricular zone of the cerebral cortex Mpl expression occurred only during late gestation, while in the hippocampus Mpl expression was detectable for first time at stage P4. In the white matter of the cerebellum Mpl expression was restricted to the perinatal period. In the adult cerebellum, Mpl expression switched to Purkinje cell. The majority of other Mpl-positive cells were NeuN-positive neurons. None of the cells could be double-labelled with astrocyte marker GFAP. Mpl
-/- mice showed no gross abnormalities of the brain.
Conclusions
Our data locate Mpl expression to neurons at different subdivisions of the spinal cord, rhombencephalon, midbrain and prosencephalon. Besides neuronal cells Mpl protein is also expressed in Purkinje cells of the adult cerebellum.
Publisher
Springer Science and Business Media LLC
Reference29 articles.
1. Noguchi CT, Asavaritikrai P, Teng R, Jia Y: Role of erythropoietin in the brain. Crit Rev Oncol Hematol. 2007, 64: 159-71. 10.1016/j.critrevonc.2007.03.001.
2. Li B, Dai W: Thrombopoietin and neurotrophins share a common domain. Blood. 1995, 86: 1643-4.
3. Ehrenreich H, Hasselblatt M, Knerlich F, von Ahsen N, Jacob S, Sperling S, Woldt H, Vehmeyer K, Nave KA, Siren AL: A hematopoietic growth factor, thrombopoietin, has a proapoptotic role in the brain. Proc Natl Acad Sci USA. 2005, 102: 862-7. 10.1073/pnas.0406008102.
4. Columbyova L, Loda M, Scadden DT: Thrombopoietin receptor expression in human cancer cell lines and primary tissues. Cancer Res. 1995, 55: 3509-12.
5. Li B, Pan H, Winkelmann JC, Dai W: Thrombopoietin and its alternatively spliced form are expressed in human amygdala and hippocampus. Blood. 1996, 87: 5382-4.
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