Examination of the endocrine-disrupting properties of “active chlorine generated from seawater by electrolysis” in response to the European Biocidal Products Regulation: current knowledge and methodological challenges
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Published:2023-10-27
Issue:1
Volume:35
Page:
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ISSN:2190-4715
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Container-title:Environmental Sciences Europe
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language:en
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Short-container-title:Environ Sci Eur
Author:
Kinani Said,Achawi Salma,Ronga-Pezeret Sylvaine,Piotrowski Aleksandra,Gauthey-Lapize Christine,Tonk Ilse,Wenker Mira,Nesslany Fabrice
Abstract
Abstract
Background
Currently, active chlorine is considered the most effective treatment for preventing biofouling of structures in contact with seawater. This compound falls under the scope of the EU Biocidal Products Regulation, which includes since 2018 a requirement to assess all active ingredients for their potential endocrine-disrupting properties on humans and non-target organisms. Therefore, this study examines the endocrine-disrupting (ED) potential of active chlorine based on the European Chemicals Agency and European Food Safety Authority guidance (ED TGD). It includes two approaches: (i) a systematic literature review using appropriate search terms and (ii) an in silico assessment, both supported by expert judgement. Finally, the feasibility and relevance of in vitro tests were examined by considering the stability of chlorine and the applicability domain of the recommended in vitro assays.
Results
No significant adversity or endocrine activity based on EATS (estrogen, androgen, thyroid, and steroidogenesis)-modalities were evidenced based on the literature data. However, these modalities remain understudied and further datasets are needed for a comprehensive assessment. The in silico approach revealed a low probability of binding between active chlorine and a set of 14 human nuclear receptors, for both agonist and antagonist effects. This is not surprising given the great structural difference between active chlorine and natural ligands. The in vitro investigation of the ED potential of active chlorine raises several operational limits, including: (i) its instability (t1/2 < 48 h) which is incompatible with a reasonable time window between collection and ex situ analysis; (ii) its rapid and complete reaction with several essential nutrients in cell culture media; (iii) its documented cytotoxicity on various cell lines; and (iv) its exclusion from the scope of certain OECD guidelines.
Conclusions
Overall, neither the in silico evaluation nor the systematic literature review performed indicates a significant adversity based on EATS-mediated parameters or EATS-related endocrine activities. This study highlights the challenges of performing a comprehensive ED assessment for a data-poor chemical and questions the relevance of transposing generic methodologies to the case of unstable and inorganic molecules.
Publisher
Springer Science and Business Media LLC
Reference91 articles.
1. Nebot E, Casanueva JF, Solera R, Pendón C, Taracido LJ, Casanueva-Robles T, López Galindo C (2010) Marine biofouling in heat exchangers. In: Chan J, Wong S (eds) Biofouling: types, impact and anti-fouling. Nova Science Publishers, New York, pp 65–104 2. Gizer G, Önal U, Ram M, Sahiner N (2023) Biofouling and mitigation methods. Biointerface Res Appl Chem 13(2):1–25 3. Walker ME, Safari I, Theregowda RB, Hsieh MK, Abbasian J, Arastoopour H, Dzombak DA, Miller DC (2012) Economic impact of condenser fouling in existing thermoelectric power plants. Energy 44:429–437 4. Pugh S, Hewitt G, Müller-Steinhagen H (2005) Fouling during the use of seawater as coolant—the development of a user guide. Heat Transf Eng 26:35–43 5. Lin H, Huang Y, Lin Y, Zhang S, Yu S, Liu K, Mou J, Lin J, He X, Fu S, Xie W, Li Z (2023) Biofouling characteristics in Xinghua Bay of Fujian, China. Front Mar Sci 9:1–12
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