Author:
Haddad Mathieu,Cimpoia Ruxandra,Zhao Ya,Guiot Serge R
Abstract
Abstract
Carboxydothermus hydrogenoformans is a thermophilic anaerobic strain most widely known for its ability to produce hydrogen (H2) when grown on carbon monoxide (CO). Although relatively well studied, growth characterization on pyruvate has never been assessed. The present work fully characterizes growth of the bacterium on pyruvate as a sole carbon source. C. hydrogenoformans demonstrated a growth rate of 0.03 h-1, with pyruvate consumption ranging between 0.21 and 0.48 mol · g-1 volatile suspended solid · d-1. A lag phase was also observed when switching from pyruvate to CO. When grown simultaneously on pyruvate and CO, pyruvate consumption was initiated upon CO depletion. This was attributed to pyruvate oxidation inhibition by CO, and not to a diauxic phenomenom. The strain also showed homoacetogenic activity.
Publisher
Springer Science and Business Media LLC
Subject
Applied Microbiology and Biotechnology,Biophysics
Reference28 articles.
1. Aono S, Honma Y, Ohkubo K, Tawara T, Kamiya T, Nakajima H: CO sensing and regulation of gene expression by the transcriptional activator CooA. J Inorg Biochem 2000,82(1–4):51–56.
2. Blamey JM, Adams MWW: Purification and characterization of pyruvate ferredoxin oxidoreductase from the hyperthermophilic archaeon Pyrococcus furiosus . Biochim Biophys Acta 1993,1161(1):19–27. 10.1016/0167-4838(93)90190-3
3. Bock A-K, Schonheit P: Growth of Methanosarcina barkeri (Fusaro) under nonmethanogenic conditions by the fermentation of pyruvate to acetate: ATP synthesis via the mechanism of substrate level phosphorylation. J Bacteriol 1995,177(8):2002–2007.
4. Carere C, Kalia V, Sparling R, Cicek N, Levin DB: Pyruvate catabolism and hydrogen synthesis pathway genes of Clostridium thermocellum ATCC 27405. Indian J Microbiol 2008,48(2):252–266. 10.1007/s12088-008-0036-z
5. Eaton AD, Clesceri LS, Rice EW, Greenberg AE: Standard methods for the examination of water and wastewater. 21st edition. Washington, D.C: American Public Health Association, American Water Works Association, Water Environment Federation; 2005.
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