Author:
Arai Teppei,Koganei Kasumi,Umemura Sara,Kojima Ryo,Kato Jun,Kasumi Takafumi,Ogihara Jun
Abstract
Abstract
A fungal strain, Penicillium purpurogenum IAM 15392, produced the azaphilone Monascus pigment homolog when cultured in a medium composed of soluble starch, ammonium nitrate, yeast extract, and citrate buffer, pH 5.0. One of the typical features of violet pigment PP-V [(10Z)-12- carboxyl-monascorubramine] is that pyranoid oxygen is replaced with nitrogen. In this study, we found that glutamine synthetase (glnB) and glutamate dehydrogenase (gdh1) genes were expressed in the culture conditions conducive to PP-V production. Gln and Glu both support PP-V biosynthesis, but PP-V biosynthesis was much more efficient with Gln. We determined that synthesis of Gln by glutamine synthetase from ammonium is important for PP-V production.
Publisher
Springer Science and Business Media LLC
Subject
Applied Microbiology and Biotechnology,Biophysics
Reference43 articles.
1. Akihisa T, Tokuda H, Ukiya M, Kiyota A, Yasukawa K, Sakamoto N, Kimura Y, Suzuki T, Takayasu J, Nishino H: Anti-tumor-initiating effects of monascin, an azaphilonoid pigment fromthe extract of Monascus pilosus fermented rice (Red-mold rice). Chem Biodivers 2005, 2: 1305–1309. 10.1002/cbdv.200590101
2. Akihisa T, Tokuda H, Yasukawa K, Ukiya M, Kiyota A, Sakamoto N, Suzuki T, Tanabe N, Nishino H: Azaphilones, furanoisophthalides, and amino acids from the extracts of Monascus pilosus -fermented rice (Red-mold rice) and their chemopreventive effects. J Agric Food Chem 2005, 53: 562–565. 10.1021/jf040199p
3. Arai T, Umemura S, Ota T, Ogihara J, Kato J, Kasumi T: Effects of inorganic nitrogen sources on the production of PP-V [(10Z)-12-carboxyl-monascorubramine] and the expression of the nitrate assimilation gene cluster by Penicillium sp. AZ. Biosci Biotechnol Biochem 2012,76(1):120–124. 10.1271/bbb.110589
4. Birch AJ, Cassera A, Fitton P, Holker JS, Smith H, Tomsor GA, Walley WB: Studies in relation to biosynthesis. Part XXX. Rotation, monascin and rubropuntatin. J Chem Soc 1962, 3583–3592. 10.1039/JR9620003583
5. Blane PJ, Loret MO, Goma G: Production of citrinin by various species of Monascus . Biotechnol Lett 1995, 17: 291–294. 10.1007/BF01190639
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