Author:
Torigoe Kenta,Torigoe Miki,Oka Satoru,Obata Yoko,Mukae Hiroshi,Nishino Tomoya
Abstract
Abstract
Background and objective
Aspartame (l-aspartyl l-phenylalanine methyl ester) is an artificial sweetener widely used as a sugar substitute. There are concerns regarding the effects of high aspartame doses on the kidney owing to oxidative stress; however, whether the maximum allowed dose of aspartame in humans affects the kidneys remains unknown. Therefore, in this study, we investigated whether the maximum allowed dose of aspartame in humans affects the kidneys.
Methods
In this study, animals were fed a folate-deficient diet to mimic human aspartame metabolism. Eight-week-old ICR mice were divided into control (CTL), 40 mg/kg/day of aspartame-administered (ASP), folate-deficient diet (FD), and 40 mg/kg/day of aspartame-administered with a folate-deficient diet (FD + ASP) groups. Aspartame was administered orally for eight weeks. Thereafter, we evaluated aspartame’s effect on kidneys via histological analysis.
Results
There were no differences in serum creatinine and blood urea nitrogen levels between the CTL and ASP groups or between the FD and FD + ASP groups. There was no histological change in the kidneys in any group. The expression of superoxide dismutase and 4-hydroxy-2-nonenal in the kidney did not differ between the CTL and ASP groups or the FD and FD + ASP groups.
Conclusion
Our findings indicate that the allowed doses of aspartame in humans may not affect kidney function or oxidative states.
Publisher
Springer Science and Business Media LLC
Reference29 articles.
1. Czarnecka K, Pilarz A, Rogut A, Maj P, Szymańska J, Olejnik Ł et al. Aspartame-true or false? Narrative review of safety analysis of general use in products. Nutrients. 2021;13:1957. https://doi.org/10.3390/nu13061957.
2. Belpoggi F, Soffritti M, Padovani M, Degli Esposti D, Lauriola M, Minardi F. Results of long-term carcinogenicity bioassay on Sprague-Dawley rats exposed to Aspartame administered in feed. Ann N Y Acad Sci. 2006;1076:559–77. https://doi.org/10.1196/annals.1371.080.
3. Landrigan PJ, Straif K. Aspartame and cancer - new evidence for causation. Environ Health. 2021;20:42. https://doi.org/10.1186/s12940-021-00725-y.
4. EFSA Panel on Food Additives and Nutrient Sources added to Food (ANS). Scientific opinion on the re-evaluation of aspartame (E 951) as a food additive. EFSA J. 2013;11:3496.
5. Ardalan MR, Tabibi H, Ebrahimzadeh Attari V, Malek Mahdavi A. Nephrotoxic effect of Aspartame as an artificial sweetener: a brief review. Iran J Kidney Dis. 2017;11:339–43.