Author:
Ito Sakae,Nagata Mayumi,Takino Yuka,Ishigami Akihito,Senoo Tadashi
Abstract
Abstract
Objective
The stability of ascorbic acid (AA) in the human aqueous humor (AqH) remains unclear. This study aimed to investigate the stability of AqH AA under varying conditions (27, 4, − 20, and − 80 °C) without acidification.
Results
Rapid AA degradation occurred at 27 °C. At 4 °C, a significant 12.2% degradation was observed after 24 h. Storage at − 20 °C resulted in a notable 37.5% degradation after 28 days, whereas storage at − 80 °C resulted in 10.7% degradation after 28 days. Unacidified AqH samples recorded early decomposition at 27 °C and 4 °C. In conclusion, it is recommended to conduct measurements within 28 days for samples stored at − 80 °C.
Funder
the Scholarship Fund for Young/Women Researchers from the Promotion and Mutual Aid Corporation for Private Schools of Japan
Publisher
Springer Science and Business Media LLC