Author:
Jin Pei-Yin,Lu Yong-Chao,Li Ling,Han Qin-Fu
Abstract
Abstract
Background
Ovarian hyper stimulation syndrome (OHSS) is an iatrogenic complication associated with fertility drugs. It is characterized by increased vascular permeability and substantial fluid shift with accumulation in the body cavity. The pathogenesis of OHSS remains obscure, and no definitive treatments are currently available.
Results
Using western blot and short-circuit current (Isc) techniques, we investigate the potential coactions of analysis in cystic fibrosis transmembrane conductance regulator (CFTR) and aquaporin 1 (AQP1) on the hyper permeability of body cavity peritoneal epithelial cells in the pathogenesis of OHSS. The rats develop OHSS symptoms, with the up regulation of both CFTR and AQP1 expression and enhanced CFTR channel activity in peritoneal epithelial cells, can also be mimicked by administration of estrogen, alone in ovariectomized rats. Administration of progesterone suppresses CFTR activity, OHSS symptoms as well as CFTR and AQP1 expression. Besides, AQP1 inhibitor, HgCl2, can suppress CFTR channel activity. Therefore, antisera against CFTR or AQP1 to OHSS animals may result in alleviation of the symptom.
Conclusion
This study confirms the coactions of CFTR and AQP1 play a critical role in the development and progression of increased peritoneal epithelial permeability in severe OHSS. These findings may provide grounds for ameliorating assisted reproduction treatment strategy to reduce the risk of OHSS in in vitro fertilization (IVF).
Publisher
Springer Science and Business Media LLC
Reference36 articles.
1. Navot D, Bergh PA, Laufer N: Ovarian hyper stimulation syndrome in novel reproductive technologies: prevention and treatment. Fertil Steril. 1992, 58: 249-261.
2. Balasch J, Fabregues F, Arroyo V: Peripheral arterial vasodilation hypothesis: a new insight into the pathogenesis of ovarian hyper stimulation syndrome. Hum Reprod. 1998, 13: 271-273.
3. Navot D, Bergh PA, Laufer N: The ovarian hyper stimulation syndrome. Reproductive endocrinology, surgery and technology. Edited by: Adashi EY, Rock JA, Rosenwaks Z. 1995, Lippincott-Raven, Philadelphia, 2215-2232.
4. Pellicer A, Albert C, Mercader A, Bonilla-Musoles F: Remohı´ J, Simo´n C: The pathogenesis of ovarian hyper stimulation syndrome: in vivo studies investigating the role of interleukin (IL)-1β, IL-6 and vascular endothelial growth factor (VEFG). Fertil Steril. 1999, 71: 482-489. 10.1016/S0015-0282(98)00484-1.
5. Go´mez R, Simo´n C, Remohı´ J, Pellicer A: Vascular endothelial growth factor receptor-2 activation induces vascular permeability in hyperstimulated rats, and this effect is prevented by receptor blockade. Endocrinology. 2002, 143: 4339-4348. 10.1210/en.2002-220204.
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