Complement C3 mediates podocyte injury through TLR4/NFΚB-P65 signaling during ischemia–reperfusion acute kidney injury and post-injury fibrosis-Reference-Cited by-同舟云学术

Complement C3 mediates podocyte injury through TLR4/NFΚB-P65 signaling during ischemia–reperfusion acute kidney injury and post-injury fibrosis

Published:2023-03-27 Issue:1 Volume:28 Page:
ISSN:2047-783X
Container-title:European Journal of Medical Research
language:en
Short-container-title:Eur J Med Res

Author:

Chen Yi,Lin Liyu,Rao Siyi,Tao Xuan,Cui Jiong,Wan Jianxin

Abstract

Abstract Background The aim of this study was to explore the mechanism of complement C3a mediating podocyte injury during ischemia–reperfusion acute kidney injury (IR-AKI) and post-injury fibrosis. Methods Renal artery clamping was used to establish IR-AKI and post-injury fibrosis model. HE and Masson staining were performed to observe renal fibrosis. The protein abundance levels were measured along with inflammatory markers, renal complement C3. Podocytes were treated with C3a with or without Toll-like receptor 4(TLR4) inhibitor. The effects of TLR4 up-regulation by TLR4 plasmids were examined. Results C3−/− resulted in amelioration of renal dysfunction by reducing podocyte damage and renal fibrosis. Immunoblot with renal tissue homogenates from IR-AKI mice revealed that C3−/− decreased TLR4/Nuclear Factor-κB (NFκB)-P65. Conclusion Our results indicate that modulating C3/TLR4/NFκB-P65 signaling pathway is a novel therapeutic target for the IR-AKI and post-injury fibrosis. Graphical Abstract

Publisher

Springer Science and Business Media LLC

Subject

General Medicine

Link

https://link.springer.com/content/pdf/10.1186/s40001-023-01054-1.pdf

Reference40 articles.

1. GBD 2019 Diseases and Injuries Collaborators. Global burden of 369 diseases and injuries in 204 countries and territories, 1990–2019: a systematic analysis for the Global Burden of Disease Study 2019. Lancet. 2020;2020(396):1204–22. https://doi.org/10.1016/S0140-6736(20)30925-9.

2. Zhang L, Wang F, Wang L, Wang W, Liu B, Liu J, Chen M, He Q, Liao Y, Yu X, Chen N, Zhang JE, Hu Z, Liu F, Hong D, Ma L, Liu H, Zhou X, Chen J, Pan L, Chen W, Wang W, Li X, Wang H. Prevalence of chronic kidney disease in China: a cross-sectional survey. Lancet. 2012;379:815–22. https://doi.org/10.1016/S0140-67.

3. Fang Y, Teng J, Ding X. Acute kidney injury in China. Hemodial Int. 2015;19(2–10):2015. https://doi.org/10.1111/hdi.12193.

4. Zhang L, Zhao MH, Zuo L, Wang Y, Yu F, Zhang H, Wang H. China Kidney Disease Network (CK-NET) 2015 Annual Data Report. Kidney Int Suppl. 2019;9:e1–81. https://doi.org/10.1016/j.kisu.2018.11.001.

5. Zhou M, Wang H, Zeng X, Yin P, Zhu J, Chen W, Li X, Wang L, Wang L, Liu Y, Liu J, Zhang M, Qi J, Yu S, Afshin A, Gakidou E, Glenn S, Krish VS, Miller-Petrie MK, Mountjoy-Venning WC, Mullany EC, Redford SB, Liu H, Naghavi M, Hay SI, Wang L, Murray CJL, Liang X. Mortality, morbidity, and risk factors in China and its provinces, 1990–2017: a systematic analysis for the Global Burden of Disease Study 2017. Lancet. 2019;394:1145–58.

Cited by 2 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Complement activation in wasp venom-induced acute kidney injury;Renal Failure;2024-04-21

2. Saikosaponin D mitigate pilocarpine‐induced astrocyte injury by regulating the NLRP3/caspase‐1 signaling pathway;Chemical Biology & Drug Design;2024-03