Author:
Zhou Zhenhai,Luo Hao,Yu Honggui,Liu Zhiming,Zhong Junlong,Xiong Jiachao,Cao Kai
Abstract
Abstract
Background
To seek the potential therapy for spinal cord injury, Ferrostatin-1, the first ferroptosis inhibitor, was administrated in spinal cord injury mice to identify the therapeutic effect.
Methods
Spinal cord injury model was established by a modified Allen’s method. Then, ferrostatin-1 was administrated by intraspinal injection. Cortical evoked motor potential and BMS were indicated to assess the neurological function rehabilitation. H&E, Nissl’s staining, NeuN, and GFAP immunofluorescence were used to identify the histological manifestation on the mice with the injured spinal cord. Spinosin, a selective small molecule activator of the Nrf2/HO-1 signaling pathway, was administrated to verify the underlying mechanism of ferrostatin-1.
Results
Ferrostatin-1 promoted the rehabilitation of cortical evoked motor potential and BMS scores, synchronized with improvement in the histological manifestation of neuron survival and scar formation. Spinosin disturbed the benefits of ferrostatin-1 administration on histological and neurobehavioral manifestation by deranging the Nrf2/HO-1 signaling pathway.
Conclusions
Ferrostatin-1 improved the rehabilitation of spinal cord injury mice by regulating ferroptosis through the Nrf2/HO-1 signaling pathway.
Funder
Natural Science Foundation of Jiangxi Province
5511 Innovation-driven Program of Jiangxi Province Department of Science and Technology
Clinical Cultivation Project of the First Affiliated Hospital of Nanchang University
National Natural Science Foundation of China
Key Project of Natural Science Foundation of Jiangxi Province
Major Discipline Academic and Technical Leaders Training Program of Jiangxi Province
Double-thousand Plan Program of Jiangxi Province
Publisher
Springer Science and Business Media LLC