Author:
Jiang Yushen,Zhang Shanming,Qin Hong,Meng Shuai,Deng Xuyi,Lin He,Xin Xiaoliang,Liang Yuxin,Chen Bowen,Cui Yan,Su YiHeng,Liang Pei,Zhou GuangZhi,Hu Hongbo
Abstract
Abstract
Background
The outbreak of novel coronavirus disease 2019 (COVID-19) has become a public health emergency of international concern. Quantitative testing of SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) virus is demanded in evaluating the efficacy of antiviral drugs and vaccines and RT-PCR can be widely deployed in the clinical assay of viral loads. Here, we developed a quantitative RT-PCR method for SARS-CoV-2 virus detection in this study.
Methods
RT-PCR kits targeting E (envelope) gene, N (nucleocapsid) gene and RdRP (RNA-dependent RNA polymerase) gene of SARS-CoV-2 from Roche Diagnostics were evaluated and E gene kit was employed for quantitative detection of COVID-19 virus using Cobas Z480. Viral load was calculated according to the standard curve established by series dilution of an E-gene RNA standard provided by Tib-Molbiol (a division of Roche Diagnostics). Assay performance was evaluated.
Results
The performance of the assay is acceptable with limit of detection (LOD) below 10E1 copies/μL and lower limit of quantification (LLOQ) as 10E2 copies/μL.
Conclusion
A quantitative detection of the COVID-19 virus based on RT-PCR was established.
Publisher
Springer Science and Business Media LLC
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