Author:
Othma Amr Abulhamd Sayed,Gomaa Howayda Ezz Eldin,El Anany Mervat Gaber,Abdul Rahman Eiman Mohammed,Hassan Eman Mahmoud,Abd Elbaky Abeer M. Nour Eldin,Soliman May Mohamed Sherif,Awadallah Eman
Abstract
AbstractBackgroundDiarrhoea is still a major public health issue in developing countries, and it is one of the leading causes of morbidity and mortality in children. We aimed to assess the use of a multiplex reverse transcription polymerase chain reaction (RT-PCR) assay forthe detection of five viruses, includingrotavirus, norovirus (genogroups 1 and 2), astrovirus, and adenovirus, responsible for gastroenteritis in children under 5 years old in primary care centres in Upper Egypt.Subjects and methodsA total of 500 stool samples were collected. Fifty samples were randomly selected for viral examination using multiplex RT-PCRfor the detection of rotavirus, norovirus (genogroups 1 and 2), astrovirus, and adenovirus, causing diarrhoea.ResultsViruses were detected in 45 (90%) of the 50 stool samples. The most frequently identified virus was norovirus G2, followed by Group A rotavirus, astrovirus and adenovirus. Mixed infection by two and three viruses was observed in 7/50 cases (14%) and 2/50 cases (4%), respectively. Norovirus G1 was not detected in the samples examined.ConclusionOur study reveals that multiplex PCR allows for the detection of multiple viral targets in only one reaction, rendering the assay easier to perform compared to existing testing methodologies (RT-PCR and electron microscopy). Additionally, most of the viruses were detected in summer, and the highest prevalence was in the age group less than 1 year. Norovirus G2 and rotavirus were the most frequent agents and the most common coinfections responsible for gastroenteritis in children.
Publisher
Springer Science and Business Media LLC
Reference44 articles.
1. https://www.who.int/news-room/fact-sheets/detail/diarrhoeal-disease (2017)
2. Groome MJ (2019) Understanding the full clinical spectrum of childhood diarrhoea in low-income and middle-income countries. Lancet Glob Health 7(5):E534–E535
3. GBD 2016 Diarrheal Disease Collaborators (2018) Estimates of the global, regional, and national morbidity, mortality, and etiologies of diarrhea in 195 countries: a systematic analysis for the Global Burden of Disease Study 2016. Lancet Infect Dis 18:1211–28
4. Nguyen GT, Phan K, Teng I, Pu J, Watanabe T (2017) Systematic review and meta-analysis of the prevalence of norovirus in cases of gastroenteritis in developing countries. Medicine 96(40):e8139. https://doi.org/10.1097/MD.0000000000008139
5. Payne DC, Vinjé J, Szilagyi PG, Edwards KM, Staat MA, Weinberg GA, Wikswo M (2013) Norovirus and medically attended gastroenteritis in US children. NEJM 368(12):1121–1130
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