Author:
Kong Deshenyue,Mao Jun-Hong,Li Hong,Wang Jian-Yu,Li Yu-Yang,Wu Xiao-Cong,Re Guo-Fen,Luo Hua-You,Kuang Yi-Qun,Wang Kun-Hua
Abstract
AbstractBackgroundMethamphetamine (METH) abuse causes serious health problems, including injury to the immune system, leading to increased incidence of infections and even making withdrawal more difficult. Of course, immune cells, an important part of the immune system, are also injured in methamphetamine abuse. However, due to different research models and the lack of bioinformatics, the mechanism of METH injury to immune cells has not been clarified.MethodsWe examined the response of three common immune cell lines, namely Jurkat, NK-92 and THP-1 cell lines, to methamphetamine by cell viability and apoptosis assay in vitro, and examined their response patterns at the mRNA level by RNA-sequencing. Differential expression analysis of two conditions (control and METH treatment) in three types of immune cells was performed using the DESeq2 R package (1.20.0). And some of the differentially expressed genes were verified by qPCR. We performed Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis of differentially expressed genes by the clusterProfiler R package (3.14.3). And gene enrichment analysis was also performed using MetaScape (www.metascape.org).ResultsThe viability of the three immune cells was differentially affected by methamphetamine, and the rate of NK-cell apoptosis was significantly increased. At the mRNA level, we found disorders of cholesterol metabolism in Jurkat cells, activation of ERK1 and ERK2 cascade in NK-92 cells, and disruption of calcium transport channels in THP-1 cells. In addition, all three cells showed changes in the phospholipid metabolic process.ConclusionsThe results suggest that both innate and adaptive immune cells are affected by METH abuse, and there may be commonalities between different immune cells at the transcriptome level. These results provide new insights into the potential effects by which METH injures the immune cells.
Funder
Special Project for Famous Doctor in Yunnan Province
Science and Technology Department of Yunnan Province
Yunnan Fundamental Research Projects
"Exosomal Mir-125a regulates TNF-α-mediated repair of intestinal barrier injury in heroin addicts during withdrawal" from the Central Public Welfare Research Institute
the "brain-gut axis" to study the role and mechanism of intestinal flora in drug addiction truncation
Fund for Yunling Scholar
Fund of Department of Education of Yunnan Province
Major Scientific and Technological Projects (biomedicine); the biomedical science resource database of the First Hospital affiliated to Kunming Medical University
Publisher
Springer Science and Business Media LLC
Subject
Genetics (clinical),Genetics
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献