Author:
Tang Le,Zhou Yu-Jie,Zhu Songling,Liang Gong-Da,Zhuang He,Zhao Man-Fei,Chang Xiao-Yun,Li Hai-Ning,Liu Zheng,Guo Zhi-Rong,Liu Wei-Qiao,He Xiaoyan,Wang Chun-Xiao,Zhao Dan-Dan,Li Jia-Jing,Mu Xiao-Qin,Yao Bing-Qing,Li Xia,Li Yong-Guo,Duo Li-Bo,Wang Li,Johnston Randal N.,Zhou Jin,Zhao Jing-Bo,Liu Gui-Rong,Liu Shu-Lin
Abstract
Abstract
Background
Escherichia coli are mostly commensals but also contain pathogenic lineages. It is largely unclear whether the commensal E. coli as the potential origins of pathogenic lineages may consist of monophyletic or polyphyletic populations, elucidation of which is expected to lead to novel insights into the associations of E. coli diversity with human health and diseases.
Methods
Using genomic sequencing and pulsed field gel electrophoresis (PFGE) techniques, we analyzed E. coli from the intestinal microbiota of three groups of healthy individuals, including preschool children, university students, and seniors of a longevity village, as well as colorectal cancer (CRC) patients, to probe the commensal E. coli populations for their diversity.
Results
We delineated the 2280 fresh E. coli isolates from 185 subjects into distinct genome types (genotypes) by PFGE. The genomic diversity of the sampled E. coli populations was so high that a given subject may have multiple genotypes of E. coli, with the general diversity within a host going up from preschool children through university students to seniors. Compared to the healthy subjects, the CRC patients had the lowest diversity level among their E. coli isolates. Notably, E. coli isolates from CRC patients could suppress the growth of E. coli bacteria isolated from healthy controls under nutrient-limited culture conditions.
Conclusions
The coexistence of multiple E. coli lineages in a host may help create and maintain a microbial environment that is beneficial to the host. As such, the low diversity of E. coli bacteria may be associated with unhealthy microenvironment in the intestine and hence facilitate the pathogenesis of diseases such as CRC.
Funder
National Natural Science Foundation of China
National Postdoctoral Fellowship of China
Heilongjiang Innovation Endowment Award for graduate studies
Publisher
Springer Science and Business Media LLC
Subject
Genetics (clinical),Genetics
Cited by
7 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献