AgNP-PVP-meglumine antimoniate nanocomposite reduces Leishmania amazonensis infection in macrophages

Author:

Gélvez Ana Patricia Cacua,Diniz Junior José Antonio Picanço,Brígida Rebecca Thereza Silva Santa,Rodrigues Ana Paula Drummond

Abstract

Abstract Background Leishmaniasis is an infectious disease caused by parasites of the genus Leishmania and presents different clinical manifestations. The adverse effects, immunosuppression and resistant strains associated with this disease necessitate the development of new drugs. Nanoparticles have shown potential as alternative antileishmanial drugs. We showed in a previous study the biosynthesis, characterization and ideal concentration of a nanocomposite that promoted leishmanicidal activity. In the present study, we conducted a specific analysis to show the mechanism of action of AgNP-PVP-MA (silver nanoparticle–polyvinylpyrrolidone-[meglumine antimoniate (Glucantime®)]) nanocomposite during Leishmania amazonensis infection in vitro. Results Through ultrastructural analysis, we observed significant alterations, such as the presence of small vesicles in the flagellar pocket and in the extracellular membrane, myelin-like structure formation in the Golgi complex and mitochondria, flagellum and plasma membrane rupture, and electrodense material deposition at the edges of the parasite nucleus in both evolutive forms. Furthermore, the Leishmania parasite infection index in macrophages decreased significantly after treatment, and nitric oxide and reactive oxygen species production levels were determined. Additionally, inflammatory, and pro-inflammatory cytokine and chemokine production levels were evaluated. The IL-4, TNF-α and MIP-1α levels increased significantly, while the IL-17 A level decreased significantly after treatment. Conclusions Thus, we demonstrate in this study that the AgNP-PVP-MA nanocomposite has leishmanial potential, and the mechanism of action was demonstrated for the first time, showing that this bioproduct seems to be a potential alternative treatment for leishmaniasis.

Publisher

Springer Science and Business Media LLC

Subject

Microbiology (medical),Microbiology

Reference65 articles.

1. Organización Panamericana de la Salud (OPAS). Manual de procedimientos para la vigilancia y control de las leishmaniasis en las Américas. Washington, D.C.: OPS; 2019.

2. World Health Organization (WHO). Leishmaniasis. Guide of the WHO/PAHO Expert Committee on the Control of Leishmaniases. Epidemiological Report of the Americas Nº 7. Washington, D.C: World Health Organization; 2019. https://iris.paho.org/bitstream/handle/10665.2/50505/Leishreport2019_eng.pdf?ua=1

3. Palumbo E. Treatment strategies for mucocutaneous leishmaniasis. J Global Infect Dis. 2010;2(2): https://doi.org/10.4103/0974-777x.62879.

4. Mcgwire BS, Satoskar AR, Leishmaniasis. Clinical syndromes and treatment. QJM. 2014;107(1):7–14.

5. Anversa LS, Tiburcio MG, Richini-Pereira VB, Ramirez LE. Human leishmaniasis in Brazil: a general review. Rev Assoc Med Bras. 2018;64(3):281–9.

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3