Endophytic microbiome of Boehmeria nivea and their antagonism against latent fungal pathogens in plants

Abstract

AbstractBackgroundPathogenic microbes still become obstacles that can reduce the quality of plant growth, including ramie (Boehmeria nivea) plants. The study identified the microbiome and antagonistic interaction of the endophytic community from theB. niveais necessary to improve the production of the ramie plant, especially ramie stem organs for fiber materials. ResultsTwenty isolates of endophytic microorganisms were obtained from the roots, stems, leaves, and flowers. They were identified using the Internal Transcribed Spacer (ITS) region of ribosomal (rDNA), and its morphotypes obtained 20 isolates, with a composition of 9 species of bacteria and 11 species of fungi. Besides that, the disease observations on ramie stems showed that four species of pathogenic fungi were identified asFusarium solaniisolate 3,248,941,Fusarium solaniisolates colpat-359,Fusarium oxysporumisolate N-61–2,Clonostachys roseastrain B3042. The endophytic microorganism of ramie ability was tested to determine their potential to inhibit the growth of the pathogenic fungi based on the in-vivo antagonist test. The isolated bacteria were only able to inhibit the growth ofF. solani, with the highest percentage of 54–55%. Three species of endophytic fungi, includingCladosporium tennissimum, Fusarium falciforme, and Penicillium citrinum, showed the best inhibition against the fungal pathogenFusarium solaniwith the highest inhibitory presentation of 91–95%. Inhibitory interaction between the endophytic microbes and the ramie pathogens indicated the type of antibiosis, competition, and parasitism. ConclusionThe results of this study succeeded in showing the potential antifungal by endophytic fungi from ramie against the pathogens of the plant itself.P. citrinum isolate MEBP0017 showed the highest inhibition against all the pathogens of the ramie.