Author:
Moghaddam Sina,Nojoomi Farshad,Dabbagh Moghaddam Arasb,Mohammadimehr Mojgan,Sakhaee Fatemeh,Masoumi Morteza,Siadat Seyed Davar,Fateh Abolfazl
Abstract
Abstract
Purpose
Nontuberculous mycobacteria (NTM) are ubiquitous bacteria that are naturally resistant to disinfectants and antibiotics and can colonize systems for supplying drinking water. Therefore, this study aimed to evaluate the prevalence of NTM in the drinking water of six hospitals in Tehran, Iran.
Methods
Totally, 198 water samples were collected. Each water sample was filtered via a membrane filter with a pore size of 0.45 µm and then decontaminated by 0.005% cetylpyridinium chloride. The membrane filters were incubated on two Lowenstein-Jensen media at 25 °C and 37 °C for 8 weeks. The positive cultures were identified with phenotypic tests, and then NTM species were detected according to the hsp65, rpoB, and 16S rDNA genes. Drug susceptibility testing (DST) was also carried out.
Results
Overall, 76 (40.4%) of the isolates were slowly growing mycobacteria (SGM) and 112 (59.6%) of the ones were rapidly growing mycobacteria (RGM). The most common NTM were Mycobacterium aurum, M. gordonae, M. phocaicum, M. mucogenicum, M. kansasii, M. simiae, M. gadium, M. lentiflavum, M. fortuitum, and M. porcinum. Among these 188 samples, NTM ranged from 1 to > 300 colony-forming unit (CFU) /500 mL, with a median of 182 CFU/500 mL. In the infectious department of all hospitals, the amount of CFU was higher than in other parts of the hospitals. The DST findings in this study indicated the diversity of resistance to different drugs. Among RGM, M. mucogenicum was the most susceptible isolate; however, M. fortuitum showed a different resistance pattern. Also, among SGM isolates, M. kansasii and M. simiae, the diversity of DST indicated.
Conclusions
The current study showed NTM strains could be an important component of hospital water supplies and a possible source of nosocomial infections according to the CFU reported in this study. The obtained findings also help clarify the dynamics of NTM variety and distribution in the water systems of hospitals in the research area.
Publisher
Springer Science and Business Media LLC
Subject
Microbiology (medical),Microbiology
Reference37 articles.
1. Collier SA, Deng L, Adam EA, Benedict KM, Beshearse EM, Blackstock AJ, Bruce BB, Derado G, Edens C, Fullerton KE. Estimate of burden and direct healthcare cost of infectious waterborne disease in the United States. Emerg Infect Dis. 2021;27(1):140.
2. Ratnatunga CN, Lutzky VP, Kupz A, Doolan DL, Reid DW, Field M, Bell SC, Thomson RM, Miles JJ. The rise of non-tuberculosis mycobacterial lung disease. Frontiers Immunol. 2020;11:303.
3. Blanc S, Robinson D, Fahrenfeld N. Potential for nontuberculous mycobacteria proliferation in natural and engineered water systems due to climate change: A literature review. City and Environment Interactions. 2021;11:100070.
4. Falkinham JO 3rd. Ecology of nontuberculous mycobacteria--where do human infections come from? Semin Respir Crit Care Med. 2013;34(1):95-102.
5. Allen M, Antwi-Agyei P, Aragon-Durand F, Babiker M, Bertoldi P, Bind M, Brown S, Buckeridge M, Camilloni I, Cartwright A. Technical Summary: Global Warming of 1.5 °C. An IPCC Special Report on the Impacts of Global Warming of 1.5 °C above pre-Industrial Levels and Related Global Greenhouse Gas Emission Pathways, in the Context of Strengthening the Global Response to the Threat of Climate Change, Sustainable Development, and Efforts to Eradicate Poverty. Geneve, Switzerland: Intergovernmental Panel on Climate Change; 2019.
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