Analysis of gut microbiota of ladybug beetle (Harmonia axyridis) after feeding on different artificial diets
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Published:2024-01-03
Issue:1
Volume:24
Page:
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ISSN:1471-2180
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Container-title:BMC Microbiology
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language:en
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Short-container-title:BMC Microbiol
Author:
Xie Bing-Hua,Chao Lei,Wan Si-Jing,Si Hui-Ru,Yu Wei-Dong,Huang Zhen,Wang Shi-Gui,Desneux Nicolas,Tang Bin,Sun Si-Si
Abstract
Abstract
Background
Harmonia axyridis is an effective natural enemy insect to a variety of phloem-sucking pests and Lepidopteran larvae, such as aphids, scabies, and phylloxera, while its industrial production is limited due to unmature artificial diet. Insect intestinal microbiota affect host development and reproduction. The aim of this study is to understand intestinal microbiota composition of H. axyridis and screen effective probiotics on artificial diet. Considering the role of the components and composition of the diet on the structure and composition of the intestinal microbiome, four kinds of diets were set up: (1) aphid; (2) basic diet; (3) basic diet + glucose; (4) basic diet + trehalose. The gut microbiota of H. axyridis was detected after feeding on different diets.
Results
Results showed that the gut microbiota between artificial diet group and aphid groups were far apart, while the basic and glucose groups were clearly clustered. Besides, the glucose group and trehalose group had one unique phylum, Cryptophyta and Candidatus Saccharibacteria, respectively. The highest abundance of Proteobacteria was found in the aphid diet. The highest abundance of Firmicutes was found in the basic diet. However, the addition of glucose or trehalose alleviated the change. In addition, the relative abundance of Enterobacter, Klebsiella, Enterobacteriaceae_unclassified, Enterobacteriales_unclassified and Serratia in the aphid group was higher than other groups. Moreover, the function of gut genes in each group also showed clear differences.
Conclusion
These results have offered a strong link between artificial diets and gut microbes, and also have provided a theoretical basis for the screening of synergistic probiotics in artificial diet.
Funder
Hangzhou Science and Technology Development Program of China National Natural Science Foundation of China National Key Research and Development Program of China Scientific and Technological Support Program from the Guizhou Branch of China National Tobacco Corporation
Publisher
Springer Science and Business Media LLC
Subject
Microbiology (medical),Microbiology
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