Author:
Fauzia Kartika Afrida,Aftab Hafeza,Miftahussurur Muhammad,Waskito Langgeng Agung,Tuan Vo Phuoc,Alfaray Ricky Indra,Matsumoto Takashi,Yurugi Michiyuki,Subsomwong Phawinee,Kabamba Evariste Tshibangu,Akada Junko,Yamaoka Yoshio
Abstract
Abstract
Background
Infection with Helicobacter pylori as the cause of gastric cancer is a global public health concern. In addition to protecting germs from antibiotics, biofilms reduce the efficacy of H. pylori eradication therapy. The nucleotide polymorphisms (SNPs) related with the biofilm forming phenotype of Helicobacter pylori were studied.
Results
Fifty-six H. pylori isolate from Bangladeshi patients were included in this cross-sectional study. Crystal violet assay was used to quantify biofilm amount, and the strains were classified into high- and low-biofilm formers As a result, strains were classified as 19.6% high- and 81.4% low-biofilm formers. These phenotypes were not related to specific clades in the phylogenetic analysis. The accessories genes associated with biofilm from whole-genome sequences were extracted and analysed, and SNPs among the previously reported biofilm-related genes were analysed. Biofilm formation was significantly associated with SNPs of alpA, alpB, cagE, cgt, csd4, csd5, futB, gluP, homD, and murF (P < 0.05). Among the SNPs reported in alpB, strains encoding the N156K, G160S, and A223V mutations were high-biofilm formers.
Conclusions
This study revealed the potential role of SNPs in biofilm formation and proposed a method to detect mutation in biofilm from whole-genome sequences.
Funder
Japan Agency for Medical Research and Development (AMED) [e-ASIA JRP, Science and Technology Research Partnership for Sustainable Development (SATREPS), Global Alliance for Chronic Diseases (GACD)]
Japanese Government (MEXT) scholarship program
Ministry of Education, Culture, Sports, Science and Technology
National Institutes of Health
Japan International Cooperation Agency (JICA) [SATREPS]
Thailand Science Research and Innovation Fundamental Fund, Bualuang ASEAN Chair Professorship at Thammasat University, and Center of Excellence in Digestive Diseases, Thammasat University, Thailand
Publisher
Springer Science and Business Media LLC
Subject
Microbiology (medical),Microbiology
Cited by
2 articles.
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