Author:
Legesse Abinet,Mekuriaw Aregitu,Gelaye Esayas,Abayneh Takele,Getachew Belayneh,Weldemedhin Wubet,Tesgera Takele,Deresse Getaw,Birhanu Kenaw
Abstract
Abstract
Background
Brucellosis is an economically devastating animal disease and has public health concern. Serological methods such as Rose Bengal Plate Test (RBPT), Complement Fixation Test (CFT), and Indirect-Enzyme-Linked Immunosorbent Assay (I-ELISA) have been used to detect brucellosis. However, there is limited comparative evaluation studies and lack of molecular confirmation of the causative agents in the study areas. The study was aimed to compare RBPT, I-ELISA, CFT, and confirmation using Polymerase Chain Reaction (PCR). A total of 2317 sera samples were collected from brucellosis-affected areas of Ethiopia with no vaccination history. All sera were subjected to comparative serological assays. Post-cross tabulation, sensitivity, and specificity were determined using Receiver Operating Characteristics (ROC) curve analysis software. PCR was performed on 54 seropositive samples using genus- and species-specific primers.
Results
Among the 2317 sera tested for comparative serological assays, 189 (8.16%) were positive for RBPT, 191 (8.24%) for I-ELISA, and 48 (2.07%) for CFT. Sensitivity to RBPT was 100% (95%) in shoats and 74% (95%) in cattle. Specificity on RBPT was 98.69% (95%), 99.28% (95%), 100% (95%) in sheep, goats, and cattle, respectively. CFT sensitivity was 4 (95%) in sheep, 9.65 (95%) goats, and 72 (95%) cattle. Specificity on CFT was 100% (95%) for sheep, goats, and cattle. A 223bp Brucella genus-specific and 156bp B. abortus species-specific detected. However, B. melitensis not detected.
Conclusion
In this study, I-ELISA was the most sensitive and specific test. RBPT detected all Brucellosis-infected sheep and goats; nevertheless, it showed false positive in sheep and goats and false negative in cattle. The presence of B. abortus in small and large ruminants was confirmed by PCR. This is the first report of B. abortus detection in small ruminant in Ethiopia. B.abortus detected in non-preferred hosts. The findings suggest further study on molecular epidemiology of Brucella species.
Publisher
Springer Science and Business Media LLC
Subject
Microbiology (medical),Microbiology
Reference41 articles.
1. Ficht TA. Brucella taxonomy and evolution. Future Microbiol. 2010;5:859–66.
2. Smits HL, Kadri SM. Brucellosis in India: a deceptive infectious disease. Indian J Med Res. 2005;122(5):375.
3. Robinson A. Guidelines for coordinated human and animal brucellosis surveillance (FAO animal production and health paper 156). Rome: Food and Agriculture Organization; 2003.
4. Gessese AT, Mulate B, Nazir S, Asmare AJ. Seroprevalence of brucellosis in camels (Camelus dromedaries) in South East Ethiopia. J Veterinary Sci Med Diagnosis. 2014;3(1):2.
5. Adugna KE, Agga GE, Zewde GJ. Seroepidemiological survey of bovine brucellosis in cattle under a traditional production system in western Ethiopia. Revolution of Science and Technology. 2013;32(3):765–3.
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