Neutralization of the Staphylococcus aureus Panton-Valentine leukocidin by African and Caucasian sera

Author:

Grebe Tobias,Rudolf Viktoria,Gouleu Christiane Sidonie,Löffler Bettina,Adegnika Ayola Akim,Shittu Adebayo Osagie,Deinhardt-Emmer Stefanie,Niemann Silke,Schaumburg Frieder

Abstract

Abstract Background The prevalence of Staphylococcus aureus isolates carrying the Panton-Valentine leukocidin (PVL) gene is higher in Africa (≈50%) compared to Europe (< 5%). The study aimed to measure anti-PVL-antibodies in Africans and Germans in a multi-center study and to test whether detected antibodies can neutralize the cytotoxic effect of PVL on polymorphonuclear leukocytes (PMNs). Methods Sera from asymptomatic Africans (n = 22, Nigeria, Gabon) and Caucasians (n = 22, Germany) were used to quantify antibody titers against PVL and α-hemolysin (in arbitrary units [AU]) by ELISA. PMNs from one African and German donor were exposed to 5 nM recombinant PVL to measure the neutralizing effect of serial dilutions of pooled sera from African and Caucasian participants, or donor sera at 0.625 and 2.5% (v/v). Results Anti-PVL-antibodies were significantly higher in Africans than in Germans (1.9 vs. 0.7 AU, p < 0.0001). The pooled sera from the study participants neutralized the cytotoxic effect of PVL on African and German PMNs in a dose dependent manner. Also, neutralization of PVL on PMNs from the African and German donors had a stronger effect with African sera (half-maximal inhibitory concentration (IC50) = 0.27 and 0.47%, respectively) compared to Caucasian sera (IC50 = 3.51 and 3.59% respectively). Conclusion Africans have higher levels of neutralizing anti-PVL-antibodies. It remains unclear if or at what level these antibodies protect against PVL-related diseases.

Funder

Westfälische Wilhelms-Universität Münster

Publisher

Springer Science and Business Media LLC

Subject

Microbiology (medical),Microbiology

Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Microbial keratitis in Southern Malawi: a microbiological pilot study;BMJ Open Ophthalmology;2024-04

2. Correspondence;Deutsches Ärzteblatt international;2023-05-30

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