Author:
Maeda Sachi,Ohka Fumiharu,Okuno Yusuke,Aoki Kosuke,Motomura Kazuya,Takeuchi Kazuhito,Kusakari Hironao,Yanagisawa Nobuyuki,Sato Shinya,Yamaguchi Junya,Tanahashi Kuniaki,Hirano Masaki,Kato Akira,Shimizu Hiroyuki,Kitano Yotaro,Yamazaki Shintaro,Yamashita Shinji,Takeshima Hideo,Shinjo Keiko,Kondo Yutaka,Wakabayashi Toshihiko,Natsume Atsushi
Abstract
AbstractDiffuse midline glioma, H3 K27M-mutant is a lethal brain tumor located in the thalamus, brain stem, or spinal cord. H3 K27M encoded by the mutation of a histone H3 gene such as H3F3A plays a pivotal role in the tumorigenesis of this type of glioma. Although several studies have revealed comprehensive genetic and epigenetic profiling, the prognostic factors of these tumors have not been identified to date. In various cancers, oncogenic driver genes have been found to exhibit characteristic copy number alterations termed mutant allele specific imbalance (MASI). Here, we showed that several diffuse midline glioma, H3 K27M-mutant exhibited high variant allele frequency (VAF) of the mutated H3F3A gene using droplet digital polymerase chain reaction (ddPCR) assays. Whole-genome sequencing (WGS) revealed that these cases had various copy number alterations that affected the mutant and/or wild-type alleles of the H3F3A gene. We also found that these MASI cases showed a significantly higher Ki-67 index and poorer survival compared with those in the lower VAF cases (P < 0.05). Our results indicated that the MASI of the H3F3A K27M mutation was associated with the aggressive phenotype of the diffuse midline glioma, H3 K27M-mutant via upregulation of the H3 K27M mutant protein, resulting in downregulation of H3K27me3 modification.
Funder
Japan Society for the Promotion of Science
Publisher
Springer Science and Business Media LLC
Subject
Cellular and Molecular Neuroscience,Neurology (clinical),Pathology and Forensic Medicine
Cited by
16 articles.
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