Oils with different degree of saturation: effects on ileal digestibility of fat and corresponding additivity and bacterial community in growing pigs

Abstract

Abstract Background Oils are important sources of energy in pig diets. The combination of oils with different degree of saturation contributes to improve the utilization efficiency of the mixed oils and may reduce the cost of oil supplemented. An experiment was conducted to evaluate the effects of oils with different degree of saturation on the fat digestibility and corresponding additivity and bacterial community in growing pigs. Methods Eighteen crossbred (Duroc × Landrace × Yorkshire) barrows (initial body weight: 29.3 ± 2.8 kg) were surgically fitted with a T-cannula in the distal ileum. The experimental diets included a fat-free basal diet and 5 oil-added diets. The 5 oil-added diets were formulated by adding 6% oil with different ratio of unsaturated to saturated fatty acids (U:S) to the basal diet. The 5 oils were palm oil (U:S = 1.2), canola oil (U:S = 12.0), and palm oil and canola oil were mixed in different proportions to prepare a combination of U:S of 2.5, 3.5 and 4.5, respectively. Results The apparent and standardized ileal digestibility (AID and SID) of fat and fatty acids increased linearly (P < 0.05) as the U:S of dietary oils increased except for SID of fat and C18:2. The AID and SID of fat and fatty acids differed among the dietary treatments (P < 0.05) except for SID of unsaturated fatty acids (UFA) and C18:2. Fitted one-slope broken-line analyses for the SID of fat, saturated fatty acids (SFA) and UFA indicated that the breakpoint for U:S of oil was 4.14 (R2 = 0.89, P < 0.01), 2.91 (R2 = 0.98, P < 0.01) and 3.84 (R2 = 0.85, P < 0.01), respectively. The determined SID of fat, C18:1, C18:2 and UFA in the mixtures was not different from the calculated SID of fat, C18:1, C18:2 and UFA. However, the determined SID of C16:0, C18:0 and SFA in the mixtures were greater than the calculated SID values (P < 0.05). The abundance of Romboutsia and Turicibacter in pigs fed diet containing palm oil was greater than that in rapeseed oil treatment group, and the two bacteria were negatively correlated with SID of C16:0, C18:0 and SFA (P < 0.05). Conclusions The optimal U:S for improving the utilization efficiency of mixed oil was 4.14. The SID of fat and UFA for palm oil and canola oil were additive in growing pigs, whereas the SID of SFA in the mixture of two oils was greater than the sum of the values of pure oils. Differences in fat digestibility caused by oils differing in degree of saturation has a significant impact on bacterial community in the foregut.