Author:
Hermansen Russell A.,Mannakee Brian K.,Knecht Wolfgang,Liberles David A.,Gutenkunst Ryan N.
Abstract
Abstract
Background
Selection on proteins is typically measured with the assumption that each protein acts independently. However, selection more likely acts at higher levels of biological organization, requiring an integrative view of protein function. Here, we built a kinetic model for de novo pyrimidine biosynthesis in the yeast Saccharomyces cerevisiae to relate pathway function to selective pressures on individual protein-encoding genes.
Results
Gene families across yeast were constructed for each member of the pathway and the ratio of nonsynonymous to synonymous nucleotide substitution rates (dN/dS) was estimated for each enzyme from S. cerevisiae and closely related species. We found a positive relationship between the influence that each enzyme has on pathway function and its selective constraint.
Conclusions
We expect this trend to be locally present for enzymes that have pathway control, but over longer evolutionary timescales we expect that mutation-selection balance may change the enzymes that have pathway control.
Funder
National Science Foundation
Publisher
Springer Science and Business Media LLC
Subject
Ecology, Evolution, Behavior and Systematics
Cited by
14 articles.
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