Genes associated with hot defensive bee ball in the Japanese honeybee, Apis cerana japonica

Abstract

AbstractBackgroundThe Japanese honeybee,Apis cerana japonica, shows a specific defensive behavior, known as a “hot defensive bee ball,” used against the giant hornet,Vespa mandarinia. Hundreds of honeybee workers surround a hornet and make a “bee ball” during this behavior. They maintain the ball for around 30 min, and its core temperature can reach 46. Although various studies have been conducted on the characteristics of this behavior, its molecular mechanism has yet to be elucidated. Here, we performed a comprehensive transcriptomic analysis to detect candidate genes related to balling behavior.ResultsThe expression levels of differentially expressed genes (DEGs) in the brain, flight muscle, and fat body were evaluated during ball formation and incubation at 46 °C. The DEGs detected during ball formation, but not in response to heat, were considered important for ball formation. The expression of genes related to rhodopsin signaling were increased in all tissues during ball formation. DEGs detected in one or two tissues during ball formation were also identified.ConclusionsGiven that rhodopsin is involved in temperature sensing inDrosophila, the rhodopsin-related DEGs inA. cerana japonicamay be involved in temperature sensing specifically during ball formation.