The 2009 pandemic (H1N1) viruses isolated from pigs show enhanced pathogenicity in mice
-
Published:2013-06-11
Issue:1
Volume:44
Page:
-
ISSN:1297-9716
-
Container-title:Veterinary Research
-
language:en
-
Short-container-title:Vet Res
Author:
Li Yongtao,Zou Wei,Jia Guangmin,Ke Jianjiang,Zhu Jiping,Lin Xian,Zhou Hongbo,Jin Meilin
Abstract
Abstract
Since the emergence of the 2009 pandemic (H1N1) virus (2009/H1N1) in April 2009, cases of transmission from humans to pigs have been reported frequently. In our previous studies, four 2009/H1N1 variants were isolated from pigs. To better understand the phenotypic differences of the pig isolates compared with the human isolate, in this study mice were inoculated intranasally with different 2009/H1N1 viruses, and monitored for morbidity, mortality, and viral replication, cytokine production and pathological changes in the lungs. The results show that all isolates show effective replication in lungs, but varying in their ability to cause morbidity. In particular, the strains of A/swine/Nanchang/3/2010 (H1N1) and A/swine/Nanchang/F9/2010 (H1N1) show the greatest virulence with a persisting replication in lungs and high lethality for mice, compared with the human isolate A/Liaoning /14/2009 (H1N1), which shows low virulence in mice. Furthermore, the lethal strains could induce more severe lung pathological changes and higher production of cytokines than that of other strains at an early stage. Amino acid sequence analysis illustrates prominent differences in viral surface glycoproteins and polymerase subunits between pig isolates and human strains that might correlate with their phenotypic differences. These studies demonstrate that the 2009/H1N1 pig isolates exhibit heterogeneous infectivity and pathogencity in mice, and some strains possess an enhanced pathogenicity compared with the human isolate.
Publisher
Springer Science and Business Media LLC
Subject
General Veterinary
Reference39 articles.
1. Dawood FS, Jain S, Finelli L, Shaw MW, Lindstrom S, Garten RJ, Gubareva LV, Xu X, Bridges CB, Uyeki TM: Emergence of a novel swine-origin influenza A (H1N1) virus in humans. N Engl J Med. 2009, 360: 2605-2615. 2. Forgie SE, Keenliside J, Wilkinson C, Webby R, Lu P, Sorensen O, Fonseca K, Barman S, Rubrum A, Stigger E, Marrie TJ, Marshall F, Spady DW, Hu J, Loeb M, Russell ML, Babiuk LA: Swine outbreak of pandemic influenza A virus on a Canadian research farm supports human-to-swine transmission. Clin Infect Dis. 2011, 52: 10-18. 10.1093/cid/ciq030. 3. Chen H, Wang Y, Liu W, Zhang J, Dong B, Fan X, de Jong MD, Farrar J, Riley S, Smith GJ, Guan Y: Serologic survey of pandemic (H1N1) 2009 virus, Guangxi Province, China. Emerg Infect Dis. 2009, 15: 1849-1850. 10.3201/eid1511.090868. 4. Zhou H, Wang C, Yang Y, Guo X, Kang C, Chen H, Jin M: Pandemic (H1N1) 2009 virus in swine herds, People’s Republic of China. Emerg Infect Dis. 2011, 17: 1757-1759. 10.3201/eid1709.101916. 5. Sreta D, Tantawet S, Na Ayudhya SN, Thontiravong A, Wongphatcharachai M, Lapkuntod J, Bunpapong N, Tuanudom R, Suradhat S, Vimolket L, Poovorawan Y, Thanawongnuwech R, Amonsin A, Kitikoon P: Pandemic (H1N1) 2009 virus on commercial swine farm, Thailand. Emerg Infect Dis. 2010, 16: 1587-1590. 10.3201/eid1610.100665.
Cited by
5 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|