Author:
Chaudhari Atul A,Jawale Chetan V,Kim Sam Woong,Lee John Hwa
Abstract
Abstract
In order to develop a novel, safe and immunogenic fowl typhoid (FT) vaccine candidate, a Salmonella Gallinarum ghost with controlled expression of the bacteriophage PhiX174 lysis gene E was constructed using pMMP99 plasmid in this study. The formation of the Salmonella Gallinarum ghost with tunnel formation and loss of cytoplasmic contents was observed by scanning electron microscopy and transmission electron microscopy. No viable cells were detectable 24 h after the induction of gene E expression by an increase in temperature from 37 °C to 42 °C. The safety and protective efficacy of the Salmonella Gallinarum ghost vaccine was tested in chickens that were divided into four groups: group A (non-immunized control), group B (orally immunized), group C (subcutaneously immunized) and group D (intramuscularly immunized). The birds were immunized at day 7 of age. None of the immunized animals showed any adverse reactions such as abnormal behavior, mortality, or signs of FT such as anorexia, depression, or diarrhea. These birds were subsequently challenged with a virulent Salmonella Gallinarum strain at 3 weeks post-immunization (wpi). Significant protection against the virulent challenge was observed in all immunized groups based on mortality and post-mortem lesions compared to the non-immunized control group. In addition, immunization with the Salmonella Gallinarum ghosts induced significantly high systemic IgG response in all immunized groups. Among the groups, orally-vaccinated group B showed significantly higher levels of secreted IgA. A potent antigen-specific lymphocyte activation response along with significantly increased percentages of CD4+ and CD8+ T lymphocytes found in all immunized groups clearly indicate the induction of cellular immune responses. Overall, these findings suggest that the newly constructed Salmonella Gallinarum ghost appears to be a safe, highly immunogenic, and efficient non-living bacterial vaccine candidate that protects against FT.
Publisher
Springer Science and Business Media LLC
Reference47 articles.
1. Barrow PA, Wallis TS: Vaccination of food animals. Salmonella in domestic animals. Edited by: Wray C, Wray A. 2000, CABI, Wallingford, 323-339.
2. Pomeroy BS, Nagaraja KV: Fowl Typhoid. Diseases of Poultry. Edited by: Calnek BW, Barnes HJ, Beard CW, Reid WM, Yoder HW Jr. 1991, Iowa State University Press, Ames, 87-99.
3. Basnet HB, Kwon HJ, Cho SH, Kim SJ, Yoo HS, Park YH, Yoon SI, Shin NS, Youn HJ: Reproduction of fowl typhoid by respiratory challenge with Salmonella Gallinarum. Avian Dis. 2008, 52: 156-159. 10.1637/7974-032607-Reg.
4. Wigley P, Hulme S, Powers C, Beal R, Smith A, Barrow P: Oral infection with the Salmonella enterica serovar Gallinarum 9R attenuated live vaccine as a model to characterize immunity to fowl typhoid in the chicken. BMC Vet Res. 2005, 1: 2-10.1186/1746-6148-1-2.
5. Zhang-Barber L, Turner AK, Dougan G, Barrow PA: Protection of chickens against experimental fowl typhoid using a nuoG mutant of Salmonella serotype Gallinarum. Vaccine. 1998, 16: 899-903. 10.1016/S0264-410X(97)00300-9.
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47 articles.
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