Author:
Almeida Luciana P,Trombone Ana PF,Lorenzi Julio CC,Rocha Carolina D,Malardo Thiago,Fontoura Isabela C,Gembre Ana F,Silva Ricardo LL,Silva Célio L,Castelo Ademilson P,Coelho-Castelo Arlete AM
Abstract
Abstract
Background
Although B cells are important as antigen presenting cells (APC) during the immune response, their role in DNA vaccination models is unknown.
Methods
In this study in vitro and in vivo experiments were performed to evaluate the ability of B cells to protect mice against Mycobacterium tuberculosis challenge.
Results
In vitro and in vivo studies showed that B cells efficiently present antigens after naked plasmid pcDNA3 encoding M. leprae 65-kDa heat shock protein (pcDNA3-Hsp65) internalization and protect B knock-out (BKO) mice against Mycobacterium tuberculosis infection. pcDNA3-Hsp65-transfected B cells adoptively transferred into BKO mice rescued the memory phenotypes and reduced the number of CFU compared to wild-type mice.
Conclusions
These data not only suggest that B cells play an important role in the induction of CD8 T cells but also that they improve bacterial clearance in DNA vaccine model.
Publisher
Springer Science and Business Media LLC
Subject
Molecular Medicine,Immunology,Immunology and Allergy,Biotechnology
Cited by
10 articles.
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