Author:
Villar Livia Melo,de Lima Marjorie Parra,Cruz Helena Medina,de Paula Vanessa Salete,Scalioni Leticia de Paula,Flores Geane Lopes,Carvalho-Costa Filipe Anibal,Parente Cynara Carvalho,Coelho Maria Rosangela Cunha Duarte,de Albuquerque Ana Cecilia Cavalcanti,Milagres Flavio Augusto Pádua,Cruz Marcelo Santos,Andrade Tarcisio Matos,Motta-Castro Ana Rita Coimbra,da Mota Jurema Corrêa,Lewis-Ximenez Lia Laura,Bastos Francisco Inácio
Abstract
Abstract
Background
Agile, accessible and cheap diagnosis of hepatitis C virus (HCV) infection is essential to achieve the elimination of this infection, worldwide, as mandated by the World Health Organzation as part of its strategy for 2030. Dried blood spots (DBS) can be an attractive alternative for sample collection among people living in remote areas and vulnerable populations due to the less invasive collection, its biosafety, and storage & transportation of samples at room temperature.
Design
This study aims to estimate the usefulness of dried blood spot samples for the diagnosis and the assessment of HCV infection rates in three different settings in Brazil. Cross-sectional analysis of a sample collection from different populations, aiming to assess the performance of the testing algorithms and respective procedures among different populations with diverse background infection rates.
Methods
We reported the evaluation of DBS as alternative samples for detecting anti-HCV in different groups in real life conditions: (I) Vulnerable subjects living in remote areas of Southeast, North and Northeast Brazil (n = 1464); (II) Beauticians (n = 288); (III) People who use non-injectable drugs (n = 201); (IV) patients referred to outpatient care (n = 275).
Results
General assay accuracy was 99%, with a weighted kappa value of 0.9, showing an excellent performance. Sensitivities ranged from 87.5% to 100.0% between groups and specificities were above 99.2%. A total of 194 individuals had HCV RNA in serum and concordance of anti-HCV detection in DBS was 98.4%.
Conclusions
DBS samples could be used for anti-HCV detection in different populations recruited in real life conditions and ambulatory settings, with a high overall sensitivity and specificity.
Funder
Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
Conselho Nacional de Desenvolvimento Científico e Tecnológico
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Fundação Oswaldo Cruz
Newton Fund
Publisher
Springer Science and Business Media LLC
Reference36 articles.
1. Organization WH. Hepatitis C 2021 [Fact sheet]. Available from: https://www.who.int/news-room/fact-sheets/detail/hepatitis-c.
2. Pereira LM, Martelli CM, Moreira RC, Merchan-Hamman E, Stein AT, Cardoso MR, et al. Prevalence and risk factors of Hepatitis C virus infection in Brazil, 2005 through 2009: a cross-sectional study. BMC Infect Dis. 2013;13:60.
3. Sacks-Davis R, Daraganova G, Aitken C, Higgs P, Tracy L, Bowden S, Jenkinson R, Rolls D, Pattison P, Robins G, Grebely J, Barry A, Hellard M. Hepatitis C virus phylogenetic clustering is associated with the social-injecting network in a cohort of people who inject drugs. PLoS ONE. 2012;7(10): e47335.
4. Wang Y, Tan XD, Zhou C, Zhou W, Peng JS, Ren YS, Ni ZL, Liu B, Yang F, Gao XD. Exploratory social network analysis and gene sequencing in people who inject drugs infected with hepatitis C virus. Epidemiol Infect. 2016;144(14):3080–90.
5. World Health Organization (WHO). Guidelines on hepatitis B and C testing. Genova: 2017.
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献