Author:
Mohebali Mehdi,Keshavarz Hossein,Shirmohammad Sedigheh,Akhoundi Behnaz,Borjian Alireza,Hassanpour Gholamreza,Mamishi Setareh,Mahmoudi Shima
Abstract
Abstract
Background
Direct agglutination test (DAT) as a simple, accurate and reliable method, has been widely used for serodiagnosis of visceral leishmaniasis (VL) during the last three decades. The present study is a systematic review and meta-analysis to evaluate the diagnostic accuracy of DAT for serodiagnosis of human VL.
Methods
Electronic databases, including MEDLINE (via PubMed), SCOPUS, Web of Science, SID and Mag Iran (two Persian scientific search engines) were searched from December 2004 to April 2019. We determined the pooled sensitivity and specificity rates of DAT for the diagnosis of human VL, calculated positive and negative likelihood ratios (LR+ and LR-), and constructed summary receiver operating characteristic (ROC) curves parameters across the eligible studies.
Results
Of the 2928 records identified in the mentioned electronic databases and after examining reference lists of articles, 24 articles met inclusion criteria and were enrolled in the systematic review and out of them 20 records qualified for meta-analysis. The pooled sensitivity and specificity rates of DAT was 96% [95% CI, 92–98] and 95% [CI95% 86–99], respectively. The likelihood ratio of a positive test (LR+) was found to be 21 [CI95%, 6.6–66.5] and the likelihood ratio of a negative test (LR−) was found to be 0.04 [(CI95%, 0.02–0.08]. The combined estimate of the diagnostic odds ratio for DAT was high [467 (CI95%, 114–1912]). We found that the summary receiver operating characteristic curve (SROC) is positioned near the upper left corner of the curve and the area under curve (AUC) was 0.98 (95% CI, 0.97 to 0.99).
Conclusion
Referring to our analysis, we determined that DAT can be considered as a valuable tool for the serodiagnosis of human VL with high sensitivity and specificity. As DAT is a simple, accurate and efficient serological test, it can be recommended for serodiagnosis of human VL particularly in endemic areas.
Funder
Tehran University of Medical Sciences and Health Services
Publisher
Springer Science and Business Media LLC
Reference51 articles.
1. Organization WH. Report of a meeting of the WHO expert committee on the control of Leishmaniases, Geneva, Switzerland, 22-26 March 2010. WHO Technical Report Series 2010 (949).
2. Naeem AT, Mahmoudi S, Saboui F, Hajjaran H, Pourakbari B, Mohebali M, et al. Clinical features and laboratory findings of visceral leishmaniasis in children referred to children medical center hospital, Tehran, Iran during 2004-2011. Iran J Parasitol. 2014;9(1):1–5.
3. Abdolsalehi M, Pourakbari B, Mahmoudi S, Moradzadeh M, Keshavarz H, Mamishi S. Clinical and epidemiologic features of visceral Leishmaniasis in children: a 6-year study from an Iranian referral hospital. Infect Disord Drug Targets. 2020;20(4):461–6.
4. Alvar J, Vélez ID, Bern C, Herrero M, Desjeux P, Cano J, et al. Leishmaniasis worldwide and global estimates of its incidence. PLoS One. 2012;7(5):e35671.
5. World Health Organization. WHO to implement online epidemiological surveillance for leishmaniasis, 2016. 2016.
Cited by
12 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献