Author:
Kurdrid Pavinee,Yutthanasirikul Rayakorn,Saree Sirilak,Senachak Jittisak,Saelee Monpaveekorn,Hongsthong Apiradee
Abstract
AbstractSynechocystis histidine kinase, Sll0474: Hik28, a signal protein in a two-component signal transduction system, plays a critical role in responding to a decrease in growth temperature and is also involved in nitrogen metabolism. In the present study, under combined stress from non-optimal growth temperature and nitrogen depletion, a comparative proteomic analysis of the wild type (WT) and a deletion mutant (MT) of Synechocystis histidine kinase, Sll0474: Hik28, in a two-component signal transduction system identified the specific groups of ABC transporters that were Hik28-dependent, e.g., the iron transporter, and Hik28-independent, e.g., the phosphate transporter. The iron transporter, AfuA, was found to be upregulated only in the WT strain grown under the combined stress of high temperature and nitrogen depletion. Whereas, the expression level of the phosphate transporter, PstS, was increased in both the WT and MT strains. Moreover, the location in the genome of the genes encoding Hik28 and ABC transporters in Synechocystis sp. PCC6803 were analyzed in parallel with the comparative proteomic data. The results suggested the regulation of the ABC transporters by the gene in a two-component system located in an adjacent location in the genome.
Publisher
Springer Science and Business Media LLC
Subject
Cell Biology,Molecular Biology
Reference25 articles.
1. Hongsthong A, Sirijuntarut M, Yutthanasirikul R, Senachak J, Kurdrid P, Cheevadhanarak S, et al. Subcellular proteomic characterization of the high-temperature stress response of the cyanobacterium Spirulina platensis. Proteome Sci. 2009;7(33):1–19.
2. Kurdrid P, Senachak J, Sirijuntarut M, Yutthanasirikul R, Phuengcharoen P, Jeamton W, et al. Comparative analysis of the Spirulina platensis subcellular proteome in response to low- and high-temperature stresses: uncovering cross-talk of signaling components. Proteome Sci. 2011;9(39):1–17.
3. Zavřel T, Sinetova MA, Búzová D, Literáková P, Červený J. Characterization of a model cyanobacterium Synechocystis sp. PCC 6803 autotrophic growth in a flat-panel photobioreactor. Eng Life Sci. 2014;15(1):122–32.
4. Slabas AR, Suzuki I, Murata N, Simon WJ, Hall JJ. Proteomic analysis of the heat shock response in Synechocystis PCC 6803 and a thermally tolerant knockout strain lacking the histidine kinase 34 gene. Proteomics. 2006;3(53):845–65.
5. Ibrahim IM, Puthiyaveetil S, Allen JF. A Two-Component Regulatory System in Transcriptional Control of Photosystem Stoichiometry: Redox-Dependent and Sodium Ion-Dependent Phosphoryl Transfer from Cyanobacterial Histidine Kinase Hik2 to Response Regulators Rre1 and RppA. Front Plant Sci. 2016;7(137):1–12.