Author:
Liu Yang,Niu Pingping,Zhou Mengqi,Xue Hui
Abstract
Abstract
Background
The cranial region is a complex set of blood vessels, cartilage, nerves and soft tissues. The reconstruction of cranial defects caused by trauma, congenital defects and surgical procedures presents clinical challenges. Our previous data showed that deficiency of the proteoglycan (PG) form of dentin matrix protein 1 (DMP1-PG) could lead to abnormal cranial development. In addition, DMP1-PG was highly expressed in the cranial defect areas. The present study aimed to investigate the potential role of DMP1-PG in intramembranous ossification in cranial defect repair.
Methods
Mouse cranial defect models were established by using wild- type (WT) and DMP1-PG point mutation mice. Microcomputed tomography (micro-CT) and histological staining were performed to assess the extent of repair. Immunofluorescence assays and real-time quantitative polymerase chain reaction (RT‒qPCR) were applied to detect the differentially expressed osteogenic markers. RNA sequencing was performed to probe the molecular mechanism of DMP1-PG in regulating defect healing.
Results
A delayed healing process and an abnormal osteogenic capacity of primary osteoblasts were observed in DMP1-PG point mutation mice. Furthermore, impaired inflammatory signaling pathways were detected by using RNA transcription analysis of this model.
Conclusions
Our data indicate that DMP1-PG is an indispensable positive regulator during cranial defect healing.
Publisher
Springer Science and Business Media LLC
Subject
Cell Biology,Molecular Biology
Reference49 articles.
1. desJardins-Park HE, Mascharak S, Longaker MT, Wan DC. Endogenous Mechanisms of Craniomaxillofacial Repair: Toward Novel Regenerative Therapies. Frontiers in oral health.2021; 2: 676258.
2. Behr B, Panetta NJ, Longaker MT, Quarto N. Different endogenous threshold levels of Fibroblast Growth Factor-ligands determine the healing potential of frontal and parietal bones. Bone. 2010;47(2):281–94.
3. Xue H, Tao D, Weng Y, Fan Q, Zhou S, Zhang R, et al. Glycosylation of dentin matrix protein 1 is critical for fracture healing via promoting chondrogenesis. Front Med. 2019;13(5):575–89.
4. Owston H, Giannoudis PV, Jones E. Do skeletal muscle MSCs in humans contribute to bone repair? A systematic review Injury. 2016;47(Suppl 6):S3-s15.
5. Zieba J, Munivez E, Castellon A, Jiang MM, Dawson B, Ambrose CG, et al. Fracture Healing in Collagen-Related Preclinical Models of Osteogenesis Imperfecta. 2020;35(6):1132–48.