Neurocognitive impact of Zika virus infection in adult rhesus macaques
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Published:2022-02-07
Issue:1
Volume:19
Page:
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ISSN:1742-2094
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Container-title:Journal of Neuroinflammation
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language:en
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Short-container-title:J Neuroinflammation
Author:
Hsu Denise C.ORCID, Chumpolkulwong Kesara, Corley Michael J., Hunsawong Taweewun, Inthawong Dutsadee, Schuetz Alexandra, Imerbsin Rawiwan, Silsorn Decha, Nadee Panupat, Sopanaporn Jumpol, Phuang-Ngern Yuwadee, Klungthong Chonticha, Reed Matthew, Fernandez Stefan, Ndhlovu Lishomwa C., Paul Robert, Lugo-Roman Luis, Michael Nelson L., Modjarrad Kayvon, Vasan Sandhya
Abstract
Abstract
Background
Zika virus (ZIKV) is a mosquito-transmitted flavivirus that affects many regions of the world. Infection, in utero, causes microcephaly and later developmental and neurologic impairments. The impact of ZIKV infection on neurocognition in adults has not been well described. The objective of the study was to assess the neurocognitive impact of ZIKV infection in adult rhesus macaques.
Methods
Neurocognitive assessments were performed using the Cambridge Neuropsychological Test Automated Battery (CANTAB) via a touch screen and modified Brinkman Board before and after subcutaneous ZIKV inoculation. Immune activation markers were measured in the blood and cerebral spinal fluid (CSF) by multiplex assay and flow cytometry.
Results
All animals (N = 8) had detectable ZIKV RNA in plasma at day 1 post-inoculation (PI) that peaked at day 2 PI (median 5.9, IQR 5.6–6.2 log10 genome equivalents/mL). In all eight animals, ZIKV RNA became undetectable in plasma by day 14 PI, but persisted in lymphoid tissues. ZIKV RNA was not detected in the CSF supernatant at days 4, 8, 14 and 28 PI but was detected in the brain of 2 animals at days 8 and 28 PI. Elevations in markers of immune activation in the blood and CSF were accompanied by a reduction in accuracy and reaction speed on the CANTAB in the majority of animals.
Conclusions
The co-occurrence of systemic and CSF immune perturbations and neurocognitive impairment establishes this model as useful for studying the impact of neuroinflammation on neurobehavior in rhesus macaques, as it pertains to ZIKV infection and potentially other pathogens.
Funder
henry m. jackson foundation
Publisher
Springer Science and Business Media LLC
Subject
Cellular and Molecular Neuroscience,Neurology,Immunology,General Neuroscience
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