Author:
Guo Tuanmao,Xing Yanli,Chen Zhongning,Wang Xianhong,Zhu Haiyun,Yang Lan,Yan Yong
Abstract
Abstract
Background
Growing evidence has implicated core-binding factor beta (Cbfb) as a contributor to osteoblast differentiation, which plays a key role in fracture healing. Herein, we aimed to assess whether Cbfb affects osteoblast differentiation after fibula fracture.
Methods
Initially, we established a Cbfb conditional knockout mouse model for subsequent studies. Immunohistochemical staining was conducted to detect the expression of proliferating cell nuclear antigen (PCNA) and collagen II in the fracture end. Next, we isolated and cultured osteoblasts from specific Cbfb conditional knockout mice for BrdU analysis, alkaline phosphatase (ALP) staining, and von Kossa staining to detect osteoblast viability, differentiation, and mineralization, respectively. Western blot analysis and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) were used to detect the expression of osteoblast differentiation-related genes.
Results
The Cbfb conditional knockout mice exhibited downregulated expression of PCNA and collagen II, reduced ALP activity, and mineralization, as well as diminished expression of osteoblast differentiation-related genes. Further, Cbfb knockout exerted no obvious effects on osteoblast proliferation.
Conclusions
Overall, these results substantiated that Cbfb could promote fibula fracture healing and osteoblast differentiation and thus provided a promising therapeutic target for clinical treatment of fibula fracture.
Funder
Xianyang Young and Middle-aged Science and Technology Innovation Leading Talents Project in 2018
Shaanxi Administration of Traditional Chinese Medicine Project in 2019
the Project of the Department of Science And Technology in Shaanxi Province
Key Innovation Project of Xianyang Central Hospital in 2019
Publisher
Springer Science and Business Media LLC
Subject
Orthopedics and Sports Medicine,Surgery
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