Author:
Bai Lei Peng,Lv Jia Xing,Kong Ling Wei,Cao Hai Ying,Jin Yu
Abstract
Abstract
Background
This study was aimed to explore the application value of modified closed biopsy technique in puncture biopsy of rabbit VX2 transplanted bone tumor model.
Methods
VX2 tumor was transplanted into the bilateral tibia of 30 rabbits through the tibial plateau to make the model of VX2 transplanted bone tumor. Seven days after modeling, the proximal tibia biopsy was performed under the guidance of X-ray, and the biopsy specimen was examined pathologically. The left leg was biopsied with modified closed biopsy technique (experimental group), and the right leg was biopsied with hollow needle (control group). After 14 days of modeling, all rabbits were killed after X-ray examination around the puncture hole, and the soft tissue around the puncture hole was taken for pathological examination, and the expression levels of PCNA and CD34 in the tissue extract were detected by enzyme-linked immunosorbent assay (ELISA).
Results
By the end of the experiment, a total of 3 rabbits died, and finally, 27 rabbits were included in the study. Tumor cells were detected in all the intramedullary specimens obtained by puncture biopsy. On the 14th day after modeling, X-ray showed that the occurrence rate of periosteal reaction and extraosseous high-density shadow around the puncture hole was 14.81% (4/27) in the experimental group and 40.74% (11/27) in the control group. The difference was statistically significant (P<0.05). The pathological results of soft tissue around the puncture hole showed that the tumor cell metastasis rate was 29.63% (8/27) in the experimental group and 100% (27/27) in the control group, and the difference was statistically significant (P<0.05). The expression levels of PCNA and CD34 in the experimental group were lower than those in the control group (P < 0.05).
Conclusion
Both the modified closed biopsy technique and needle aspiration biopsy can provide sufficient biopsy tissue for the diagnosis of VX2-transplanted bone tumor in rabbits. At the same time, the improved closed biopsy technique has a certain application value in preventing local metastasis of tumor cells along the puncture channel.
Funder
key project funded by the Science and Technology Department of Hebei Province, China
Publisher
Springer Science and Business Media LLC
Subject
Orthopedics and Sports Medicine,Surgery
Reference31 articles.
1. Singla A, Geller DS. Musculoskeletal tumors. Pediatr Clin North Am. 2020;67(1):227–45.
2. Karamzade ZN, Manafi FR, Ataeinia B, et al. Molecular imaging of bone metastases using tumor-targeted tracers. Q J Nucl Med Mol Im. 2019;63(2):136–49.
3. Sabattini S, Renzi A, Buracco P, et al. Comparative assessment of the accuracy of cytological and histologic biopsies in the diagnosis of canine bone lesions. J Vet Intern Med. 2017;31(3):864–71.
4. Daley NA, Reed WJ, Peterson JJ, et al. Strategies for biopsy of musculoskeletal tumors. Seminars Roentgenol. 2017;52(4):282–90.
5. Georges E, Breitburd F, Jibard N, et al. Two shope papillomavirus-associated VX2 carcinoma cell lines with different levels of keratinocyte differentiation and transplantability. J Virol. 1985;55(1):246–50.