Abstract
Abstract
Background
The commonly used laboratory rat, Rattus norvegicus, is unique in having multiple Sry gene copies found on the Y chromosome, with different copies encoding amino acid variations that influence the resulting protein function. It is not clear which Sry genes are expressed at the onset of testis differentiation or how their expression correlates with that of other genes in testis-determination pathways.
Methods
Here, two independent E11–E14 developmental RNAseq datasets show that multiple Sry genes are expressed at E12–E13.
Results
The identified copies expressed during testis initiation include Sry4A, Sry1, and Sry3C, which are conserved in every strain of Rattus norvegicus with genomes sequenced to date.
Conclusions
This work represents a first step in defining the complex environment of rat testis differentiation that can open the door for generating sex reversal model systems using embryo manipulation techniques that have been available in the mouse but not the rat.
Funder
NIH Office of the Director
National Institute of Environmental Health Sciences
David Geffen School of Medicine, University of California, Los Angeles
Publisher
Springer Science and Business Media LLC
Subject
Endocrinology,Gender Studies
Cited by
7 articles.
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