Transcriptional analysis of the multiple Sry genes and developmental program at the onset of testis differentiation in the rat

Author:

Prokop Jeremy W.,Chhetri Surya B.,van Veen J. Edward,Chen Xuqi,Underwood Adam C.,Uhl Katie,Dwinell Melinda R.,Geurts Aron M.,Correa Stephanie M.,Arnold Arthur P.ORCID

Abstract

Abstract Background The commonly used laboratory rat, Rattus norvegicus, is unique in having multiple Sry gene copies found on the Y chromosome, with different copies encoding amino acid variations that influence the resulting protein function. It is not clear which Sry genes are expressed at the onset of testis differentiation or how their expression correlates with that of other genes in testis-determination pathways. Methods Here, two independent E11–E14 developmental RNAseq datasets show that multiple Sry genes are expressed at E12–E13. Results The identified copies expressed during testis initiation include Sry4A, Sry1, and Sry3C, which are conserved in every strain of Rattus norvegicus with genomes sequenced to date. Conclusions This work represents a first step in defining the complex environment of rat testis differentiation that can open the door for generating sex reversal model systems using embryo manipulation techniques that have been available in the mouse but not the rat.

Funder

NIH Office of the Director

National Institute of Environmental Health Sciences

David Geffen School of Medicine, University of California, Los Angeles

Publisher

Springer Science and Business Media LLC

Subject

Endocrinology,Gender Studies

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